[Determination of verapamil in dog serum and tissues by reversed-phase high performance liquid chromatography]. 2004

Xin Sun, and Qingsheng Yin, and Dianliang Chen, and Xuejun Dong, and Lichun Zhou, and Hui Zhang, and Pingsheng Fan
Experimentation Center, First Affiliated Hospital, Anhui Medical University, Hefei 230022, China. liuliuwn@mail.hf.ah.cn

A method for determination of verapamil (VRPM) concentrations in dog serum and tissues was established. The study was carried out on a high performance liquid chromatograph with a reversed-phase C18 column, a mobile phase of acetate-methanol-triethylamine (40:60:1, v/v) at 1.0 mL/min, and UV detection at 228 nm. Diazepam was used as the internal standard. The detection limits of VRPM concentrations in serum and tissues were determined to be 50 microg/L and 50 ng/g, respectively. Good linearities were observed for VRPM in serum and in liver tissue (r > 0.999). The precision of the method was good and the recoveries of VRPM were higher than 90.80%. This method is rapid, accurate and convenient for determination of VRPM concentrations in serum and tissues.

UI MeSH Term Description Entries
D007202 Indicators and Reagents Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499) Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D002121 Calcium Channel Blockers A class of drugs that act by selective inhibition of calcium influx through cellular membranes. Calcium Antagonists, Exogenous,Calcium Blockaders, Exogenous,Calcium Channel Antagonist,Calcium Channel Blocker,Calcium Channel Blocking Drug,Calcium Inhibitors, Exogenous,Channel Blockers, Calcium,Exogenous Calcium Blockader,Exogenous Calcium Inhibitor,Calcium Channel Antagonists,Calcium Channel Blocking Drugs,Exogenous Calcium Antagonists,Exogenous Calcium Blockaders,Exogenous Calcium Inhibitors,Antagonist, Calcium Channel,Antagonists, Calcium Channel,Antagonists, Exogenous Calcium,Blockader, Exogenous Calcium,Blocker, Calcium Channel,Blockers, Calcium Channel,Calcium Blockader, Exogenous,Calcium Inhibitor, Exogenous,Channel Antagonist, Calcium,Channel Blocker, Calcium,Inhibitor, Exogenous Calcium
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D004285 Dogs The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065) Canis familiaris,Dog
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014700 Verapamil A calcium channel blocker that is a class IV anti-arrhythmia agent. Iproveratril,Calan,Cordilox,Dexverapamil,Falicard,Finoptin,Isoptin,Isoptine,Izoptin,Lekoptin,Verapamil Hydrochloride,Hydrochloride, Verapamil
D056148 Chromatography, Reverse-Phase A chromatography technique in which the stationary phase is composed of a non-polar substance with a polar mobile phase, in contrast to normal-phase chromatography in which the stationary phase is a polar substance with a non-polar mobile phase. Chromatography, Reversed-Phase Liquid,Reversed-Phase Chromatography,Reversed-Phase Liquid Chromatography,Reverse-Phase Chromatography,Reverse-Phase Liquid Chromatography,Chromatography, Reverse Phase,Chromatography, Reversed-Phase,Reverse Phase Chromatography,Reversed Phase Chromatography

Related Publications

Xin Sun, and Qingsheng Yin, and Dianliang Chen, and Xuejun Dong, and Lichun Zhou, and Hui Zhang, and Pingsheng Fan
April 1995, Journal of chromatography. B, Biomedical applications,
Xin Sun, and Qingsheng Yin, and Dianliang Chen, and Xuejun Dong, and Lichun Zhou, and Hui Zhang, and Pingsheng Fan
January 1985, Journal of pharmaceutical and biomedical analysis,
Xin Sun, and Qingsheng Yin, and Dianliang Chen, and Xuejun Dong, and Lichun Zhou, and Hui Zhang, and Pingsheng Fan
December 1987, Journal of chromatography,
Xin Sun, and Qingsheng Yin, and Dianliang Chen, and Xuejun Dong, and Lichun Zhou, and Hui Zhang, and Pingsheng Fan
May 2002, Se pu = Chinese journal of chromatography,
Xin Sun, and Qingsheng Yin, and Dianliang Chen, and Xuejun Dong, and Lichun Zhou, and Hui Zhang, and Pingsheng Fan
March 1985, Journal of chromatography,
Xin Sun, and Qingsheng Yin, and Dianliang Chen, and Xuejun Dong, and Lichun Zhou, and Hui Zhang, and Pingsheng Fan
September 1997, Se pu = Chinese journal of chromatography,
Xin Sun, and Qingsheng Yin, and Dianliang Chen, and Xuejun Dong, and Lichun Zhou, and Hui Zhang, and Pingsheng Fan
January 2002, Se pu = Chinese journal of chromatography,
Xin Sun, and Qingsheng Yin, and Dianliang Chen, and Xuejun Dong, and Lichun Zhou, and Hui Zhang, and Pingsheng Fan
May 1979, Journal of chromatography,
Xin Sun, and Qingsheng Yin, and Dianliang Chen, and Xuejun Dong, and Lichun Zhou, and Hui Zhang, and Pingsheng Fan
September 1988, Journal of chromatography,
Xin Sun, and Qingsheng Yin, and Dianliang Chen, and Xuejun Dong, and Lichun Zhou, and Hui Zhang, and Pingsheng Fan
November 2001, Se pu = Chinese journal of chromatography,
Copied contents to your clipboard!