[Significance of both nuclear and cytosol androgen receptor (AR) in assessment of AR status in prostate carcinoma and hepatocellular carcinoma]. 2003

Han-Ping Lu, and Jin-Hui Gao
Department of Experimental Nuclear Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou, Guangdong, 510089, PR China. hyxjys@gzsums.edu.cn

OBJECTIVE Androgen receptor (AR) is closely associated with the genesis,development,treatment and assessment of prognosis of prostate carcinoma (PC) and hepatocellular carcinoma (HCC). How to determine the AR status accurately has important clinical significance. This study was designed to investigate the significance of androgen receptor(AR) in cell nucleus for assessment of the AR status of patients with PC and HCC. METHODS A total of 94 PC and 192 HCC tissues were analyzed for the affinity (KD),cytosol AR (AcR) and nuclear AR(AnR) using radioligand binding assay(RBA). RESULTS In 94 PC tissues, the Bmax values of AcR and AnR were 58.82+/-34.73 and 543.70+/-249.44 fmol/mg protein which were significantly higher than those of the surrounding tissues (21.63+/-14.89 and 89.20+/-47.32 fmol/mg protein, P< 0.001). The KD values of AcR and AnR were 0.84+/-0.52 and 2.15+/-0.79 nmol/L which were not significantly different as compared with the surrounding tissues(0.78+/-0.49 and 2.24+/-0.84 nmol/L, P >0.50). In 192 HCC tissues, the Bmax values of AcR and AnR were 18.09+/-16.87 and 59.93+/-34.12 fmol/mg protein, which were significantly higher than those of the surrounding tissues (10.87+/-7.60 and 25.54+/-20.10 fmol/mg protein, P< 0.001 ). The KD values of AcR and AnR were 0.76+/-0.57 and 1.89+/-0.74 nmol/L)which were not significantly different as compared with the surrounding tissues(0.69+/-0.48 and 1.94+/-0.88 nmol/L, P >0.50). The ratio of AnR/AcR was also higher (P< 0.001). Of 94 PC tissues, 48.94% were both AcR and AnR positive, being lower than that of the tissues with positive AcR alone (77.66%). Of 192 HCC tissues, 34.09% were both AcR and AnR positive, being also lower than that of AcR positive alone (56.26%). However, 63.01% of PC and 62.03% of HCC AcR-positive tissues were accompanied by AnR-positive. CONCLUSIONS Despite the fact that the Bmax of both AcR and AnR increased in PC and HCC tissues as compared to the surrounding tissues, the AnR showed more significant changes. In assessment of the AR status of PC and HCC tissues, it would be more accurate to analyze with both AcR and AnR than with AcR alone.

UI MeSH Term Description Entries
D008113 Liver Neoplasms Tumors or cancer of the LIVER. Cancer of Liver,Hepatic Cancer,Liver Cancer,Cancer of the Liver,Cancer, Hepatocellular,Hepatic Neoplasms,Hepatocellular Cancer,Neoplasms, Hepatic,Neoplasms, Liver,Cancer, Hepatic,Cancer, Liver,Cancers, Hepatic,Cancers, Hepatocellular,Cancers, Liver,Hepatic Cancers,Hepatic Neoplasm,Hepatocellular Cancers,Liver Cancers,Liver Neoplasm,Neoplasm, Hepatic,Neoplasm, Liver
D008297 Male Males
D011379 Prognosis A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations. Prognostic Factor,Prognostic Factors,Factor, Prognostic,Factors, Prognostic,Prognoses
D011471 Prostatic Neoplasms Tumors or cancer of the PROSTATE. Cancer of Prostate,Prostate Cancer,Cancer of the Prostate,Neoplasms, Prostate,Neoplasms, Prostatic,Prostate Neoplasms,Prostatic Cancer,Cancer, Prostate,Cancer, Prostatic,Cancers, Prostate,Cancers, Prostatic,Neoplasm, Prostate,Neoplasm, Prostatic,Prostate Cancers,Prostate Neoplasm,Prostatic Cancers,Prostatic Neoplasm
D011944 Receptors, Androgen Proteins, generally found in the CYTOPLASM, that specifically bind ANDROGENS and mediate their cellular actions. The complex of the androgen and receptor migrates to the CELL NUCLEUS where it induces transcription of specific segments of DNA. Androgen Receptors,5 alpha-Dihydrotestosterone Receptor,Androgen Receptor,Dihydrotestosterone Receptors,Receptor, Testosterone,Receptors, Androgens,Receptors, Dihydrotestosterone,Receptors, Stanolone,Stanolone Receptor,Testosterone Receptor,5 alpha Dihydrotestosterone Receptor,Androgens Receptors,Receptor, 5 alpha-Dihydrotestosterone,Receptor, Androgen,Receptor, Stanolone,Stanolone Receptors,alpha-Dihydrotestosterone Receptor, 5
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D003600 Cytosol Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components. Cytosols
D006528 Carcinoma, Hepatocellular A primary malignant neoplasm of epithelial liver cells. It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. The cells may be uniform or markedly pleomorphic, or form GIANT CELLS. Several classification schemes have been suggested. Hepatocellular Carcinoma,Hepatoma,Liver Cancer, Adult,Liver Cell Carcinoma,Liver Cell Carcinoma, Adult,Adult Liver Cancer,Adult Liver Cancers,Cancer, Adult Liver,Cancers, Adult Liver,Carcinoma, Liver Cell,Carcinomas, Hepatocellular,Carcinomas, Liver Cell,Cell Carcinoma, Liver,Cell Carcinomas, Liver,Hepatocellular Carcinomas,Hepatomas,Liver Cancers, Adult,Liver Cell Carcinomas
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014408 Biomarkers, Tumor Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or BODY FLUIDS. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including HORMONES; ANTIGENS; amino and NUCLEIC ACIDS; ENZYMES; POLYAMINES; and specific CELL MEMBRANE PROTEINS and LIPIDS. Biochemical Tumor Marker,Cancer Biomarker,Carcinogen Markers,Markers, Tumor,Metabolite Markers, Neoplasm,Tumor Biomarker,Tumor Marker,Tumor Markers, Biochemical,Tumor Markers, Biological,Biochemical Tumor Markers,Biological Tumor Marker,Biological Tumor Markers,Biomarkers, Cancer,Marker, Biochemical Tumor,Marker, Biologic Tumor,Marker, Biological Tumor,Marker, Neoplasm Metabolite,Marker, Tumor Metabolite,Markers, Biochemical Tumor,Markers, Biological Tumor,Markers, Neoplasm Metabolite,Markers, Tumor Metabolite,Metabolite Markers, Tumor,Neoplasm Metabolite Markers,Tumor Markers, Biologic,Tumor Metabolite Marker,Biologic Tumor Marker,Biologic Tumor Markers,Biomarker, Cancer,Biomarker, Tumor,Cancer Biomarkers,Marker, Tumor,Markers, Biologic Tumor,Markers, Carcinogen,Metabolite Marker, Neoplasm,Metabolite Marker, Tumor,Neoplasm Metabolite Marker,Tumor Biomarkers,Tumor Marker, Biochemical,Tumor Marker, Biologic,Tumor Marker, Biological,Tumor Markers,Tumor Metabolite Markers

Related Publications

Han-Ping Lu, and Jin-Hui Gao
December 1998, Chinese medical journal,
Han-Ping Lu, and Jin-Hui Gao
August 1987, Experimental and clinical endocrinology,
Han-Ping Lu, and Jin-Hui Gao
July 2020, Asian journal of urology,
Han-Ping Lu, and Jin-Hui Gao
January 2001, The Journal of steroid biochemistry and molecular biology,
Han-Ping Lu, and Jin-Hui Gao
September 2012, Molecular and cellular endocrinology,
Copied contents to your clipboard!