Biomaterial Database

[An electron microscopic study of retinal development and pathogenesis in mutant CBA/J mice with hereditary retinal degeneration]. 1992

V A Poplinskaia, and O G Stroeva, and Kh Kh Semenov

Electron microscopic study of the neural retina pathogenesis was carried out on mice CBA/J in comparison with corresponding process in substrain CBA/Ki (Caley et al., 1972). A difference in terms of the pathology development was found between substrains CBA/J and CBA/Ki. It was shown that, in CBA/J, the retinal degeneration begins later than in CBA/Ki. As distinct from the latter, CBA/J proceeds to increase the thickness of outer nuclear and plexiform layers as well as the number of membranous discs of rod outer segments (ROS) between P10 and P12. In the retina of mouse CBA/J, pathologic alterations of the outer nuclear layer and the inner segments of photoreceptors precede the ROS destruction. It was found that the pigment epithelium is capable to phagocytize membranous discs of ROS in mouse CBA/J at P10 and P12. In CBA/J, the mitochondria in inner segments of photoreceptors begin to decay at P10; by P12, the amount of such segments reach 40%. At P12, the number of pycnotic nuclei (6%) in the outer nuclear layer coincides with the number of inner segments with dense degenerative cytoplasm. At P15, the state of retina as evaluated by the extent of far advanced degeneration of the outer nuclear layer is equalized in mice of both substrains. From P10 to P15, macrophages which phagocytize membranous discs of ROS are present in the interphotoreceptor space of CBA/J. The administration of PABA solution (7.5 x 10(-3) mg/g) daily from P1 to P9 or from P1 to P11 and with subsequent fixation at P10 and P12 respectively exerted no effect on the rate of retinal pathogenesis in mouse CBA/J. The causes of revealed differences in the development of retinal pathology between CBA/Ki and CBA/J are discussed as well as the causes of the absence of PABA effect on the retina of mutant mouse CBA/J.

UI	MeSH Term	Description	Entries
D008808	Mice, Inbred CBA	An inbred strain of mouse that is widely used in BIOMEDICAL RESEARCH.	Mice, CBA,Mouse, CBA,Mouse, Inbred CBA,CBA Mice,CBA Mice, Inbred,CBA Mouse,CBA Mouse, Inbred,Inbred CBA Mice,Inbred CBA Mouse
D008817	Mice, Mutant Strains	Mice bearing mutant genes which are phenotypically expressed in the animals.	Mouse, Mutant Strain,Mutant Mouse Strain,Mutant Strain of Mouse,Mutant Strains of Mice,Mice Mutant Strain,Mice Mutant Strains,Mouse Mutant Strain,Mouse Mutant Strains,Mouse Strain, Mutant,Mouse Strains, Mutant,Mutant Mouse Strains,Mutant Strain Mouse,Mutant Strains Mice,Strain Mouse, Mutant,Strain, Mutant Mouse,Strains Mice, Mutant,Strains, Mutant Mouse
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D010129	4-Aminobenzoic Acid	An aminobenzoic acid isomer that combines with pteridine and GLUTAMIC ACID to form FOLIC ACID. The fact that 4-aminobenzoic acid absorbs light throughout the UVB range has also resulted in its use as an ingredient in SUNSCREENS.	PABA,p-Aminobenzoic Acid,para-Aminobenzoic Acid,4-Aminobenzoic Acid, Potassium Salt,Aminobenzoic Acid (USP),Epit Vit,Epitelplast,Hachemina,Magnesium para-Aminobenzoate,Pabasan,Paraminan,Paraminol,Potaba,Potassium 4-Aminobenzoate,Potassium Aminobenzoate,4 Aminobenzoic Acid,4 Aminobenzoic Acid, Potassium Salt,4-Aminobenzoate, Potassium,Aminobenzoate, Potassium,Potassium 4 Aminobenzoate,p Aminobenzoic Acid,para Aminobenzoic Acid,para-Aminobenzoate, Magnesium
D010641	Phenotype	The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.	Phenotypes
D012160	Retina	The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.	Ora Serrata
D012162	Retinal Degeneration	A retrogressive pathological change in the retina, focal or generalized, caused by genetic defects, inflammation, trauma, vascular disease, or aging. Degeneration affecting predominantly the macula lutea of the retina is MACULAR DEGENERATION. (Newell, Ophthalmology: Principles and Concepts, 7th ed, p304)	Degeneration, Retinal,Degenerations, Retinal,Retinal Degenerations
D000375	Aging	The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time.	Senescence,Aging, Biological,Biological Aging
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus