Biomaterial Database

[Cloning and sequence analysis of human embryonic nasopharyngeal epithelial CYP2E1 cDNA]. 2003

De-fu Hou, and Zhi-min He, and Fang Yang

Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China.

OBJECTIVE To investigate the role of CYP2E1 gene in chemical carcinogen-induced nasopharyngeal carcinogenesis and to provide new evidence about etiology and pathogenesis of nasopharyngeal carcinoma. METHODS RT-PCR was used to clone the CYP2E1 gene in human embryonic nasopharyngeal epithelial (HENE) cell, the transformed nasopharyngeal epithelial cell line (7,429), and the nasopharyngeal carcinoma cell line (HNE1). The cloned segments were inserted into pGEM-T Easy vector to sequence by DNA recombination technique. RESULTS In comparison with HENE-2E1 cDNA, there were two point mutations at positions 846 (A to T) and 901 (A to G) in 7,429-2E1 cDNA as well as only one point mutation at position 901 (A to G) in HNE1-2E1 cDNA. In comparison with human (adult, ethanol-inducible) liver CYP2E1 gene (GenBank NO. J02843), HENE-2E1 cDNA had one point mutation at position 901 (G to A). All these point mutations didn't affect the amino acid sequence. But no base change was found in HNE1-2E1 cDNA. CONCLUSIONS There are a few of base substitutions among HENE-2E1 cDNA, 7,429-2E1 cDNA, and HNE1 cDNA sequences. All these point mutations are synonymous mutation. The study reconfirms that the human CYP2E1 gene is relatively well conserved.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009303	Nasopharyngeal Neoplasms	Tumors or cancer of the NASOPHARYNX.	Cancer of Nasopharynx,Nasopharyngeal Cancer,Cancer of the Nasopharynx,Nasopharynx Cancer,Nasopharynx Neoplasms,Neoplasms, Nasopharyngeal,Cancer, Nasopharyngeal,Cancer, Nasopharynx,Cancers, Nasopharyngeal,Cancers, Nasopharynx,Nasopharyngeal Cancers,Nasopharyngeal Neoplasm,Nasopharynx Cancers,Nasopharynx Neoplasm,Neoplasm, Nasopharyngeal,Neoplasm, Nasopharynx,Neoplasms, Nasopharynx
D009305	Nasopharynx	The top portion of the pharynx situated posterior to the nose and superior to the SOFT PALATE. The nasopharynx is the posterior extension of the nasal cavities and has a respiratory function.	Rhinopharynx,Choanae,Nasopharynges,Nasopharynxes,Rhinopharynges,Rhinopharynxes
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D004622	Embryo, Mammalian	The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.	Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D014407	Tumor Cells, Cultured	Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.	Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured
D017354	Point Mutation	A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.	Mutation, Point,Mutations, Point,Point Mutations