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The genetic characterization of ad-3 mutants of Neurospora crassa induced by two carcinogenic difunctional akylating agents, 1,2,4,5-diepoxypentane (DEP) and 1,2,7,8-diepoxyoctane (DEO), has shown that point mutations at the ad-3B locus have similar complementation patterns. In addition to the induction of point mutations, DEP induces a low frequency (7.5%) of multilocus deletions, whereas DEO induces an extremely high frequency (42.0%). The distribution of the different classes of ad-3 mutants and the frequency of multilocus deletion mutants among DEP-induced mutants are not significantly different from those induced by the monofunctional alkylating agents EI, EMS and ICR-177 at comparable forward-mutation frequencies. Moreover, the frequencies of DEP-induced ad-3B mutants showing allelic completion or having nonpolarized complementation patterns are similar to those of ad-3B mutants induced by monofunctional agents. It is suggested, therefore, that the mechanism of mutation-induction by DEP in N. crassa is similar to that of monofunctional alkylating agents. Mutation-induction by DEO probably results both from the mechanism of action of monofunctional alkylating agents and from inter-strand cross-linkage of the DNA molecular by the two functional epoxy groups.
T M Ong, and
F J Serres
January 1968,
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T M Ong, and
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April 1988,
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T M Ong, and
F J Serres
May 1975,
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T M Ong, and
F J Serres
April 1967,
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T M Ong, and
F J Serres
January 1973,
Molecular & general genetics : MGG,
T M Ong, and
F J Serres
January 1972,
Molecular & general genetics : MGG,
T M Ong, and
F J Serres
March 1980,
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T M Ong, and
F J Serres
February 1975,
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T M Ong, and
F J Serres
October 1976,
Mutation research,
T M Ong, and
F J Serres
May 1953,
Transactions of the New York Academy of Sciences,
