-transparent.png){kind=logo}
The taxonomic status of the parasitic protozoal species Giardia intestinalis depends on the morphological similarity of all Giardia isolated from humans and the presumption that Giardia are host-specific. On the basis of electrophoretic data derived from examination of 26 enzyme loci in Australian isolates, it has been proposed that G. intestinalis is a species complex comprising three or four genetically distinct (but morphologically cryptic) species. These received the tentative designations of genetic groups I-IV (R. H. Andrews, M. Adams, P. F. L. Boreham, G. Mayrhofer & B. P. Meloni. International Journal for Parasitology 19, 183-190, 1989). In the present study, two unrelated DNA probes (one specific for a gene encoding a trophozoite surface protein, the other detecting a non-coding repetitive sequence within the G. intestinalis genome) were used in Southern hybridization analyses to examine 10 axenic isolates of G. intestinalis, established from diverse geographical regions in Australia, together with the Portland-1 isolate from the USA. Both probes identified every isolate unambiguously as belonging to one or other of two genetic clusters. Electrophoretic analysis of the same samples indicated that these clusters correspond to the previously defined genetic groups I and II. No heterogeneity was apparent within the seven group I isolates using either probe. However, when probed with the repetitive sequence, the four isolates belonging to group II exhibited small differences in banding patterns, suggesting that this group may be less homogeneous than group I.(ABSTRACT TRUNCATED AT 250 WORDS)
P L Ey, and
K Khanna, and
R H Andrews, and
P A Manning, and
G Mayrhofer
January 1987,
Methods in enzymology,
P L Ey, and
K Khanna, and
R H Andrews, and
P A Manning, and
G Mayrhofer
June 1987,
Clinica chimica acta; international journal of clinical chemistry,
P L Ey, and
K Khanna, and
R H Andrews, and
P A Manning, and
G Mayrhofer
August 1994,
Genome,
P L Ey, and
K Khanna, and
R H Andrews, and
P A Manning, and
G Mayrhofer
December 1993,
Journal of Korean medical science,
P L Ey, and
K Khanna, and
R H Andrews, and
P A Manning, and
G Mayrhofer
May 1982,
American journal of human genetics,
P L Ey, and
K Khanna, and
R H Andrews, and
P A Manning, and
G Mayrhofer
May 1984,
Proceedings of the National Academy of Sciences of the United States of America,
P L Ey, and
K Khanna, and
R H Andrews, and
P A Manning, and
G Mayrhofer
January 1984,
Human genetics,
P L Ey, and
K Khanna, and
R H Andrews, and
P A Manning, and
G Mayrhofer
April 1987,
Jikken dobutsu. Experimental animals,
P L Ey, and
K Khanna, and
R H Andrews, and
P A Manning, and
G Mayrhofer
December 1988,
Applied and environmental microbiology,
P L Ey, and
K Khanna, and
R H Andrews, and
P A Manning, and
G Mayrhofer
November 1983,
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik,
