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The ability of purified T cells to be activated by immobilized anti-CD3 and soluble anti-CD2 monoclonal antibodies (mAbs) was compared using cells from young and old donors. Purified T cells from elderly humans activated with immobilized anti-CD3 mAb incorporated less [3H]thymidine (58,780 vs 92,258 cpm; p < 0.02) into cellular DNA, and secreted less IL-2 into the culture supernatants than did T cells from young donors. In contrast, T cells activated with anti-CD2 mAbs displayed no age-related differences in proliferation or IL-2 production. Anti-CD2 stimulation resulted in equal IL-2 synthesis by cells from young and old donors that was comparable to the amount produced by cells from elderly donors stimulated with immobilized anti-CD3. Northern blot analysis of early cell cycle gene expression by anti-CD2 activated T cells demonstrated no age differences in the expression of p55 IL-2R or c-myc specific mRNA, although T cells from elderly individuals activated with immobilized anti-CD3 showed statistically significant decreases in both mRNAs. T cell receptor beta chain mRNA levels did not differ between cells from young or old donors after activation by either anti-CD3 or anti-CD2. The discordance in proliferative ability, IL-2 secretion, and specific mRNA expression between T cells from elderly donors activated through the CD3-TCR complex or by soluble anti-CD2 mAbs provides additional evidence for a multifactorial causation of age-related T cell proliferative defects, and may indicate that the difference in proliferative ability is, in part, attributable to responsiveness to secreted IL-2.
L J Song, and
J E Nagel, and
F J Chrest, and
G D Collins, and
W H Adler
January 1991,
Cellular signalling,
L J Song, and
J E Nagel, and
F J Chrest, and
G D Collins, and
W H Adler
July 1988,
The EMBO journal,
L J Song, and
J E Nagel, and
F J Chrest, and
G D Collins, and
W H Adler
February 1990,
Immunology today,
L J Song, and
J E Nagel, and
F J Chrest, and
G D Collins, and
W H Adler
January 1992,
Cellular immunology,
L J Song, and
J E Nagel, and
F J Chrest, and
G D Collins, and
W H Adler
June 1991,
Journal of immunology (Baltimore, Md. : 1950),
L J Song, and
J E Nagel, and
F J Chrest, and
G D Collins, and
W H Adler
May 1989,
European journal of immunology,
L J Song, and
J E Nagel, and
F J Chrest, and
G D Collins, and
W H Adler
November 1992,
Annals of the New York Academy of Sciences,
L J Song, and
J E Nagel, and
F J Chrest, and
G D Collins, and
W H Adler
February 1992,
Proceedings of the National Academy of Sciences of the United States of America,
L J Song, and
J E Nagel, and
F J Chrest, and
G D Collins, and
W H Adler
March 1999,
Transplantation,
L J Song, and
J E Nagel, and
F J Chrest, and
G D Collins, and
W H Adler
November 1988,
The Journal of experimental medicine,
