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Human T lymphocytes can be activated through either the antigen/MHC receptor complex T3-Ti (CD3-Ti) or the T11 (CD2) molecule to proliferate via an IL-2 dependent mechanism. To investigate the relationship of these pathways to one another, we generated and characterized Jurkat mutants which selectively express either surface CD3-Ti or CD2. Here we show that CD3-Ti- mutants fail to be stimulated by either pathway to increase phosphoinositide turnover, mobilize calcium or induce the IL-2 gene. The activation capacity of these mutants via CD2 as well as CD3-Ti can be restored following reconstitution of surface CD3-Ti expression upon appropriate DNA transfer (e.g. Ti beta subunit cDNA into Ti beta- Jurkat variants). Collectively, these results demonstrate that CD3-Ti and CD2 pathways are interdependent and that phosphoinositide turnover is linked to the CD3-Ti complex.
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C Alberini, and
O Acuto, and
L K Clayton, and
C Transy, and
G C Spagnoli, and
P Moingeon, and
P Lopez, and
E L Reinherz
October 1990,
European journal of immunology,
A Alcover, and
C Alberini, and
O Acuto, and
L K Clayton, and
C Transy, and
G C Spagnoli, and
P Moingeon, and
P Lopez, and
E L Reinherz
May 1988,
Scandinavian journal of immunology,
A Alcover, and
C Alberini, and
O Acuto, and
L K Clayton, and
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G C Spagnoli, and
P Moingeon, and
P Lopez, and
E L Reinherz
February 1990,
Immunology today,
A Alcover, and
C Alberini, and
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L K Clayton, and
C Transy, and
G C Spagnoli, and
P Moingeon, and
P Lopez, and
E L Reinherz
January 2006,
Immunology,
A Alcover, and
C Alberini, and
O Acuto, and
L K Clayton, and
C Transy, and
G C Spagnoli, and
P Moingeon, and
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E L Reinherz
March 1996,
Experimental hematology,
A Alcover, and
C Alberini, and
O Acuto, and
L K Clayton, and
C Transy, and
G C Spagnoli, and
P Moingeon, and
P Lopez, and
E L Reinherz
March 2000,
Journal of investigative medicine : the official publication of the American Federation for Clinical Research,
A Alcover, and
C Alberini, and
O Acuto, and
L K Clayton, and
C Transy, and
G C Spagnoli, and
P Moingeon, and
P Lopez, and
E L Reinherz
September 1988,
Scandinavian journal of immunology,
A Alcover, and
C Alberini, and
O Acuto, and
L K Clayton, and
C Transy, and
G C Spagnoli, and
P Moingeon, and
P Lopez, and
E L Reinherz
January 1987,
Advances in experimental medicine and biology,
A Alcover, and
C Alberini, and
O Acuto, and
L K Clayton, and
C Transy, and
G C Spagnoli, and
P Moingeon, and
P Lopez, and
E L Reinherz
January 1991,
Cellular signalling,
A Alcover, and
C Alberini, and
O Acuto, and
L K Clayton, and
C Transy, and
G C Spagnoli, and
P Moingeon, and
P Lopez, and
E L Reinherz
December 1992,
Aging (Milan, Italy),
