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VP-16 resistant cells, designated VP-16K6-1 (K6-1) were isolated from human acute lymphoblastic leukemia cell line RPMI 8402. IC50 value against VP-16 were 11-fold higher than their sensitive parental cell line. The membrane permeability of the drug was not responsible for resistance in K6-1 cells. K6-1 cells showed resistance in drug-induced DNA strand breaks (single strand breaks) determined by the alkaline sucrose gradient sedimentation method. Dot-blot analysis of RNA extracted from 2 cell lines showed that the mRNA levels of DNA topoisomerase II in K6-1 cells decreased slightly compared with that of parent cells. However, Topo II activities were similar in wild-type and K6-1 cells. In addition, K6-1 cells exhibited cross-resistance only to VM-26. These data suggest that a reduced intracellular Topo II level may contribute to drug resistance as an important factor in K6-1 cells.
J H Hong, and
K Okada, and
Y Kusunoki, and
Y Komazawa, and
M Kobayashi, and
A Mizutani, and
T Mizuno, and
A Kuramoto, and
N Kamada
March 1989,
The Tohoku journal of experimental medicine,
J H Hong, and
K Okada, and
Y Kusunoki, and
Y Komazawa, and
M Kobayashi, and
A Mizutani, and
T Mizuno, and
A Kuramoto, and
N Kamada
January 1999,
Anticancer research,
J H Hong, and
K Okada, and
Y Kusunoki, and
Y Komazawa, and
M Kobayashi, and
A Mizutani, and
T Mizuno, and
A Kuramoto, and
N Kamada
December 1989,
The Tohoku journal of experimental medicine,
J H Hong, and
K Okada, and
Y Kusunoki, and
Y Komazawa, and
M Kobayashi, and
A Mizutani, and
T Mizuno, and
A Kuramoto, and
N Kamada
January 1991,
Anticancer research,
J H Hong, and
K Okada, and
Y Kusunoki, and
Y Komazawa, and
M Kobayashi, and
A Mizutani, and
T Mizuno, and
A Kuramoto, and
N Kamada
August 1985,
Cancer research,
J H Hong, and
K Okada, and
Y Kusunoki, and
Y Komazawa, and
M Kobayashi, and
A Mizutani, and
T Mizuno, and
A Kuramoto, and
N Kamada
January 2012,
Biological & pharmaceutical bulletin,
J H Hong, and
K Okada, and
Y Kusunoki, and
Y Komazawa, and
M Kobayashi, and
A Mizutani, and
T Mizuno, and
A Kuramoto, and
N Kamada
September 1986,
British journal of cancer,
J H Hong, and
K Okada, and
Y Kusunoki, and
Y Komazawa, and
M Kobayashi, and
A Mizutani, and
T Mizuno, and
A Kuramoto, and
N Kamada
December 1994,
Blood,
J H Hong, and
K Okada, and
Y Kusunoki, and
Y Komazawa, and
M Kobayashi, and
A Mizutani, and
T Mizuno, and
A Kuramoto, and
N Kamada
March 1994,
Leukemia,
J H Hong, and
K Okada, and
Y Kusunoki, and
Y Komazawa, and
M Kobayashi, and
A Mizutani, and
T Mizuno, and
A Kuramoto, and
N Kamada
November 2011,
Nucleosides, nucleotides & nucleic acids,
