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beta-Hexosaminidase (EC 3.2.1.30) is markedly increased in human serum in liver disease, chronic alcoholism, and pregnancy. Knowledge of the clearance rate of plasma/serum beta-hexosaminidase is necessary to evaluate this increase. We studied the plasma clearance of beta-hexosaminidase isoenzymes (purified from human serum and placenta) after their infusion into rat circulation. A recently developed enzyme immunoassay method was used to measure the human beta-hexosaminidase isoenzymes; this method relies on both immunoreactivity and enzyme activity, so intact human isoenzymes were measured. In comparison with the placental isoenzymes (t1/2 less than 2 min), the serum forms showed a considerably slower clearance (t1/2 = 2-4 h). However, desialylation of the serum forms resulted in their rapid clearance (t1/2 less than 2 min). The organ localization of the enzyme eliminated from the circulation was investigated 24 h after infusion. Placental and native serum isoenzymes accumulated mainly in the liver and the spleen. Desialylated serum forms were almost exclusively localized to the liver.
A Isaksson, and
B Hultberg, and
T Jonung
October 1979,
Cancer research,
A Isaksson, and
B Hultberg, and
T Jonung
June 1981,
The Biochemical journal,
A Isaksson, and
B Hultberg, and
T Jonung
January 1999,
Acta biochimica Polonica,
A Isaksson, and
B Hultberg, and
T Jonung
January 1986,
Anticancer research,
A Isaksson, and
B Hultberg, and
T Jonung
May 1981,
Endocrinology,
A Isaksson, and
B Hultberg, and
T Jonung
December 1986,
Biochemical medicine and metabolic biology,
A Isaksson, and
B Hultberg, and
T Jonung
August 2001,
Free radical biology & medicine,
A Isaksson, and
B Hultberg, and
T Jonung
January 1999,
Oncology,
A Isaksson, and
B Hultberg, and
T Jonung
January 1982,
Cancer,
A Isaksson, and
B Hultberg, and
T Jonung
September 1976,
The Biochemical journal,
