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The possibility of separating cells on the basis of levels of antigen expression was explored in a model system using fixed erythrocytes and high gradient magnetic separation (HGMS). Fixed human erythrocytes were labelled to varying degrees with tetrameric monoclonal antibody complexes specific for both dextran and glycophorin A-M. The cells were then mixed and incubated with dextran iron particles prior to magnetic separation. The small size of the dextran iron particles (less than 0.2 microns) resulted in quantitative magnetic labelling of cells as shown using fluoresceinated anti-dextran antibodies and flow cytometry. The relationships between the initial percentage of labelled cells, cell recovery, non-specific entrapment of unlabelled cells, the purity of the removed fraction, the degree of antigen expression and separation conditions (flow rate and field strength) were determined and used to establish separation conditions that allowed recovery of cells that differ only in the degree of antibody labelling.
T E Thomas, and
S J Abraham, and
A J Otter, and
E W Blackmore, and
P M Lansdorp
January 2004,
Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi,
T E Thomas, and
S J Abraham, and
A J Otter, and
E W Blackmore, and
P M Lansdorp
January 1990,
Cytometry,
T E Thomas, and
S J Abraham, and
A J Otter, and
E W Blackmore, and
P M Lansdorp
June 1981,
Cell biophysics,
T E Thomas, and
S J Abraham, and
A J Otter, and
E W Blackmore, and
P M Lansdorp
January 1977,
Bibliotheca anatomica,
T E Thomas, and
S J Abraham, and
A J Otter, and
E W Blackmore, and
P M Lansdorp
August 2016,
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
T E Thomas, and
S J Abraham, and
A J Otter, and
E W Blackmore, and
P M Lansdorp
May 1991,
Biotechnology and bioengineering,
T E Thomas, and
S J Abraham, and
A J Otter, and
E W Blackmore, and
P M Lansdorp
May 1978,
Biophysical journal,
T E Thomas, and
S J Abraham, and
A J Otter, and
E W Blackmore, and
P M Lansdorp
February 1978,
British journal of haematology,
T E Thomas, and
S J Abraham, and
A J Otter, and
E W Blackmore, and
P M Lansdorp
January 1995,
Folia biologica,
T E Thomas, and
S J Abraham, and
A J Otter, and
E W Blackmore, and
P M Lansdorp
January 2006,
Methods in molecular biology (Clifton, N.J.),
