BIOMAT Database Logo BIOMAT Database Logo

Regulation of bovine corneal endothelial cell cycle by transforming growth factor-beta. 2003

Yutaka Motegi, and Tomohiro Usui, and Kouichi Ishida, and Satoshi Kato, and Hidetoshi Yamashita
Department of Ophthalmology, University of Tokyo School of Medicine, Tokyo, Japan.

OBJECTIVE The transforming growth factor-beta (TGF-beta) family includes three multifunctional proteins, TGF-beta1, TGF-beta2 and TGF-beta3, expressed in ocular tissue, which are involved in regulating cell differentiation, cell proliferation and other cell functions. TGF-beta is present in aqueous humour and regulates corneal endothelial cells. This study explores the mechanism by which TGF-beta regulates the cell cycle in cultured corneal endothelial cells. METHODS The expression of specific receptors for the TGF-beta family was investigated at the protein level by affinity cross-linking with radio-iodinated TGF-beta1 and immunoprecipitation with specific antibodies to TGF-beta receptors. Regulation of entry into the S-phase of the cell cycle was determined by 5-bromo-2' deoxyuridine (BrdU) incorporation into the cells. The signal transduction pathways were investigated using various blocking agents for protein kinase transducers involved in intracytoplasmic signal transduction. RESULTS Cultured bovine corneal endothelial cells were confirmed to express TGF-beta type 1 and type 2 receptors and endoglin. In the confluent state, TGF-beta1 and TGF-beta2 stimulated the cells to progress to the S-phase of the cell cycle through platelet-derived growth factor-B (PDGF-B) chain production and protein kinase C. CONCLUSIONS TGF-beta accelerated cell cycle progression from the G0/G1 phase to the S-phase in cultured corneal endothelial cells, under our experimental conditions, through pathways involving protein kinase C. These pathways are related to the cross-talk between TGF-beta and other cytokines. The conditions employed in the present experiments may be useful for investigating the complex cross-talk between various cytokines and growth factors.

UI MeSH Term Description Entries
D011493 Protein Kinase C An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters. Calcium Phospholipid-Dependent Protein Kinase,Calcium-Activated Phospholipid-Dependent Kinase,PKC Serine-Threonine Kinase,Phospholipid-Sensitive Calcium-Dependent Protein Kinase,Protein Kinase M,Calcium Activated Phospholipid Dependent Kinase,Calcium Phospholipid Dependent Protein Kinase,PKC Serine Threonine Kinase,Phospholipid Sensitive Calcium Dependent Protein Kinase,Phospholipid-Dependent Kinase, Calcium-Activated,Serine-Threonine Kinase, PKC
D011956 Receptors, Cell Surface Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands. Cell Surface Receptor,Cell Surface Receptors,Hormone Receptors, Cell Surface,Receptors, Endogenous Substances,Cell Surface Hormone Receptors,Endogenous Substances Receptors,Receptor, Cell Surface,Surface Receptor, Cell
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D002453 Cell Cycle The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE. Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004728 Endothelium, Corneal Single layer of large flattened cells covering the surface of the cornea. Anterior Chamber Epithelium,Corneal Endothelium,Endothelium, Anterior Chamber,Epithelium, Anterior Chamber,Anterior Chamber Endothelium
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000071063 Endoglin A membrane glycoprotein and ANGIOGENESIS FACTOR that is expressed by cells of the VASCULAR ENDOTHELIUM; VASCULAR SMOOTH MUSCLE; and MONOCYTES. It functions as a co-receptor for TRANSFORMING GROWTH FACTOR BETA and modulates CELL ADHESION. Mutations in the endoglin gene are associated with cases of HEREDITARY HEMORRHAGIC TELANGIECTASIA. CD105 Antigen,Endoglin Protein,Antigen, CD105
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015398 Signal Transduction The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway. Cell Signaling,Receptor-Mediated Signal Transduction,Signal Pathways,Receptor Mediated Signal Transduction,Signal Transduction Pathways,Signal Transduction Systems,Pathway, Signal,Pathway, Signal Transduction,Pathways, Signal,Pathways, Signal Transduction,Receptor-Mediated Signal Transductions,Signal Pathway,Signal Transduction Pathway,Signal Transduction System,Signal Transduction, Receptor-Mediated,Signal Transductions,Signal Transductions, Receptor-Mediated,System, Signal Transduction,Systems, Signal Transduction,Transduction, Signal,Transductions, Signal

Related Publications

Yutaka Motegi, and Tomohiro Usui, and Kouichi Ishida, and Satoshi Kato, and Hidetoshi Yamashita
Endothelial cell regulation by transforming growth factor-beta.
November 1991, Journal of cellular biochemistry,
Yutaka Motegi, and Tomohiro Usui, and Kouichi Ishida, and Satoshi Kato, and Hidetoshi Yamashita
Transforming growth factor beta and cell cycle regulation.
April 1995, Cancer research,
Yutaka Motegi, and Tomohiro Usui, and Kouichi Ishida, and Satoshi Kato, and Hidetoshi Yamashita
Molecular regulation of the bovine endothelial cell nitric oxide synthase by transforming growth factor-beta 1.
August 1995, Arteriosclerosis, thrombosis, and vascular biology,
Yutaka Motegi, and Tomohiro Usui, and Kouichi Ishida, and Satoshi Kato, and Hidetoshi Yamashita
Regulation of bovine bone cell proliferation by fibroblast growth factor and transforming growth factor beta.
July 1988, Endocrinology,
Yutaka Motegi, and Tomohiro Usui, and Kouichi Ishida, and Satoshi Kato, and Hidetoshi Yamashita
Cell density-dependent regulation of proteoglycan synthesis by transforming growth factor-beta(1) in cultured bovine aortic endothelial cells.
January 2000, The Journal of biological chemistry,
Yutaka Motegi, and Tomohiro Usui, and Kouichi Ishida, and Satoshi Kato, and Hidetoshi Yamashita
Growth regulation by transforming growth factor-beta.
January 1992, Cancer treatment and research,
Yutaka Motegi, and Tomohiro Usui, and Kouichi Ishida, and Satoshi Kato, and Hidetoshi Yamashita
Transforming growth factor-beta inhibits endothelial cell proliferation.
May 1986, Biochemical and biophysical research communications,
Yutaka Motegi, and Tomohiro Usui, and Kouichi Ishida, and Satoshi Kato, and Hidetoshi Yamashita
Transforming growth factor beta regulation of cell proliferation.
January 1987, Journal of cellular physiology. Supplement,
Yutaka Motegi, and Tomohiro Usui, and Kouichi Ishida, and Satoshi Kato, and Hidetoshi Yamashita
Regulation of intestinal epithelial cell growth by transforming growth factor type beta.
March 1989, Proceedings of the National Academy of Sciences of the United States of America,
Yutaka Motegi, and Tomohiro Usui, and Kouichi Ishida, and Satoshi Kato, and Hidetoshi Yamashita
Transforming growth factor-beta and breast cancer: Cell cycle arrest by transforming growth factor-beta and its disruption in cancer.
January 2000, Breast cancer research : BCR,
Copied contents to your clipboard!