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Acidic pH plays an important role in the membrane insertion of protective antigen (PA) of anthrax toxin leading to the translocation of the catalytic moieties. The structural transitions occurring in PA as a consequence of change in pH were investigated by fluorescence and circular dichroism measurements. Our studies revealed the presence of two intermediates on-pathway of acid induced unfolding; one at pH 2.0 and other at pH 4-5. Intrinsic fluorescence measurements of these intermediates showed a red shift in the wavelength of emission maximum with a concomitant decrease in fluorescence intensity, indicative of the exposure of tryptophan residues to the bulk solvent. Furthermore, no significant change was seen in the secondary structure of PA at a pH of 2.0, as indicated by far UV-CD spectra. The low pH intermediate of PA was characterized using the hydrophobic dye, 8-anilino-1-naphthalenesulfonate, and was found to have properties similar to those of a molten globule state.
Pradeep K Gupta, and
Raj K Kurupati, and
Harish Chandra, and
Reetika Gaur, and
Vibha Tandon, and
Yogendra Singh, and
Kapil Maithal
November 2010,
Nature structural & molecular biology,
Pradeep K Gupta, and
Raj K Kurupati, and
Harish Chandra, and
Reetika Gaur, and
Vibha Tandon, and
Yogendra Singh, and
Kapil Maithal
November 2012,
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V,
Pradeep K Gupta, and
Raj K Kurupati, and
Harish Chandra, and
Reetika Gaur, and
Vibha Tandon, and
Yogendra Singh, and
Kapil Maithal
May 1981,
Veterinarni medicina,
Pradeep K Gupta, and
Raj K Kurupati, and
Harish Chandra, and
Reetika Gaur, and
Vibha Tandon, and
Yogendra Singh, and
Kapil Maithal
September 2007,
Archives of biochemistry and biophysics,
Pradeep K Gupta, and
Raj K Kurupati, and
Harish Chandra, and
Reetika Gaur, and
Vibha Tandon, and
Yogendra Singh, and
Kapil Maithal
March 2012,
Chemical biology & drug design,
Pradeep K Gupta, and
Raj K Kurupati, and
Harish Chandra, and
Reetika Gaur, and
Vibha Tandon, and
Yogendra Singh, and
Kapil Maithal
August 1999,
Biochemistry,
Pradeep K Gupta, and
Raj K Kurupati, and
Harish Chandra, and
Reetika Gaur, and
Vibha Tandon, and
Yogendra Singh, and
Kapil Maithal
April 1979,
Veterinarni medicina,
Pradeep K Gupta, and
Raj K Kurupati, and
Harish Chandra, and
Reetika Gaur, and
Vibha Tandon, and
Yogendra Singh, and
Kapil Maithal
November 1998,
Biochemistry,
Pradeep K Gupta, and
Raj K Kurupati, and
Harish Chandra, and
Reetika Gaur, and
Vibha Tandon, and
Yogendra Singh, and
Kapil Maithal
March 2010,
Analytical chemistry,
Pradeep K Gupta, and
Raj K Kurupati, and
Harish Chandra, and
Reetika Gaur, and
Vibha Tandon, and
Yogendra Singh, and
Kapil Maithal
April 1975,
Zhurnal mikrobiologii, epidemiologii i immunobiologii,
