Biomaterial Database

Electrofusion of protoplasts from Porphyra haitanensis and P. yezoensis thalli (Rhodophyta). 1992

C Chen

Xiamen Fisheries College, Jimei, China.

In this paper the electrofusion of protoplasts from P. haitanensis and P. yezoensis thalli by using an electrofusion instrument (Shimadzu Company, Japan) under different conditions of AC field, DC pulse, fusion buffer solutions and concentrations of protease are described. The results showed that the fusion rate could reach 21-31% by applying AC field of 30-35V for 25-30s and DC pulse of 300-350 V for 60 microseconds and in the presence of 3 mM Ca2+ and 3 mM Mg2+ in the fusion buffer. The pretreatment with protease had a positive effect on cell fusion. The fusion cells formed cell walls after 7 day's culture and grew into cell aggregates after 41 day's culture.

UI	MeSH Term	Description	Entries
D010450	Endopeptidases	A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.	Endopeptidase,Peptide Peptidohydrolases
D011523	Protoplasts	The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL.	Protoplast
D002021	Buffers	A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.	Buffer
D002459	Cell Fusion	Fusion of somatic cells in vitro or in vivo, which results in somatic cell hybridization.	Cell Fusions,Fusion, Cell,Fusions, Cell
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D004558	Electric Stimulation	Use of electric potential or currents to elicit biological responses.	Stimulation, Electric,Electrical Stimulation,Electric Stimulations,Electrical Stimulations,Stimulation, Electrical,Stimulations, Electric,Stimulations, Electrical
D012624	Seaweed	Multicellular marine macroalgae including some members of red (RHODOPHYTA), green (CHLOROPHYTA), and brown (PHAEOPHYCEAE) algae. They are widely distributed in the ocean, occurring from the tide level to considerable depths, free-floating (planktonic) or anchored to the substratum (benthic). They lack a specialized vascular system but take up fluids, nutrients, and gases directly from the water. They contain CHLOROPHYLL and are photosynthetic, but some also contain other light-absorbing pigments. Many are of economic importance as FOOD, fertilizer, AGAR, potash, or source of IODINE.	Macroalgae