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We developed large-scale preparation of phycoerythrin from Porphyra haitanensis, a main economic red algae in China. Firstly, P. haitanensis thallus was broken by using "swelling and smash" method. Then times of grads ammonium sulfate precipitation applied to the crude extraction were compared. Desalted solution was further purified with one-step chromatography using hydroxyapatite and properties on spectrum and molecular weight were identified finally. The results indicated that after four times of ammonium sulfate precipitation (15%, 50%, 10% and 40%), the absorption spectrum purity of P. haitanensis achieved 0.9 (A564/A280), and 507.82 mg phycoerythrin (A564/A280 > 3.2) was obtained from 7 kg fresh algae after further hydroxyapatite chromatography. This research provides a potential way for preparation of phycoerythrin in large sclae.
Chunxia Li, and
Daiyuan Yan, and
Jing Ni, and
Ziye Guo, and
Chun'er Cai, and
Peimin He
January 2013,
Biological research,
Chunxia Li, and
Daiyuan Yan, and
Jing Ni, and
Ziye Guo, and
Chun'er Cai, and
Peimin He
March 2020,
Food & function,
Chunxia Li, and
Daiyuan Yan, and
Jing Ni, and
Ziye Guo, and
Chun'er Cai, and
Peimin He
November 2020,
Food chemistry,
Chunxia Li, and
Daiyuan Yan, and
Jing Ni, and
Ziye Guo, and
Chun'er Cai, and
Peimin He
December 2020,
International journal of biological macromolecules,
Chunxia Li, and
Daiyuan Yan, and
Jing Ni, and
Ziye Guo, and
Chun'er Cai, and
Peimin He
May 2021,
Molecular nutrition & food research,
Chunxia Li, and
Daiyuan Yan, and
Jing Ni, and
Ziye Guo, and
Chun'er Cai, and
Peimin He
November 2023,
Journal of agricultural and food chemistry,
Chunxia Li, and
Daiyuan Yan, and
Jing Ni, and
Ziye Guo, and
Chun'er Cai, and
Peimin He
January 1992,
Chinese journal of biotechnology,
Chunxia Li, and
Daiyuan Yan, and
Jing Ni, and
Ziye Guo, and
Chun'er Cai, and
Peimin He
February 2020,
International journal of biological macromolecules,
Chunxia Li, and
Daiyuan Yan, and
Jing Ni, and
Ziye Guo, and
Chun'er Cai, and
Peimin He
May 2015,
Plant biology (Stuttgart, Germany),
Chunxia Li, and
Daiyuan Yan, and
Jing Ni, and
Ziye Guo, and
Chun'er Cai, and
Peimin He
June 2017,
Carbohydrate polymers,
