BIOMAT Database Logo BIOMAT Database Logo

Allelic variation in the hemolymph juvenile hormone binding protein gene of Manduca sexta. 2003

Joshua K Young, and Anthony P Orth, and Walter G Goodman
Department of Entomology, University of Wisconsin-Madison, 237 Russell Laboratory, 1630 Linden Dr., Madison, WI 53706, USA.

Peripheral distribution of the insect juvenile hormones (JHs) requires hemolymph transport proteins. A comparison of three strains of the tobacco hornworm Manduca sexta indicates that hemolymph JH binding protein (hJHBP) levels in the Madison wild-type (Mwt) strain are significantly higher than in the black larval mutant (bl) and Seattle wild-type (Swt) strains. To correlate differences in hJHBP levels between strain phenotypes with the hJHBP locus, we sequenced 8.4 kb of the hJHBP gene locus from each strain. Snb, an allele found in the Swt and bl strains, contains a 408 bp repetitive nuclear element flanked by 15 bp direct repeats. Mating studies coupled with molecular genotyping demonstrate that the presence of Snb correlates with a twofold lower hJHBP level relative to the allele found in the Mwt strain. Despite the lower hJHBP levels in individuals carrying the Snb gene, hemolymph levels of JH do not appear to be significantly affected.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D011110 Polymorphism, Genetic The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level. Gene Polymorphism,Genetic Polymorphism,Polymorphism (Genetics),Genetic Polymorphisms,Gene Polymorphisms,Polymorphism, Gene,Polymorphisms (Genetics),Polymorphisms, Gene,Polymorphisms, Genetic
D002352 Carrier Proteins Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes. Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D005838 Genotype The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS. Genogroup,Genogroups,Genotypes
D006458 Hemolymph The blood/lymphlike nutrient fluid of some invertebrates. Hemolymphs
D000483 Alleles Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product. Allelomorphs,Allele,Allelomorph
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D015139 Blotting, Southern A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES. Southern Blotting,Blot, Southern,Southern Blot
D018402 Manduca A genus of sphinx or hawk moths of the family Sphingidae. These insects are used in molecular biology studies during all stages of their life cycle. Manduca sexta,Tobacco Hornworm,Hornworm, Tobacco,Hornworms, Tobacco,Manduca sextas,Manducas,Tobacco Hornworms,sextas, Manduca

Related Publications

Joshua K Young, and Anthony P Orth, and Walter G Goodman
Photoaffinity labeling of the hemolymph juvenile hormone binding protein of Manduca sexta.
December 1984, Biochemistry,
Joshua K Young, and Anthony P Orth, and Walter G Goodman
The Juvenile Hormone Binding Protein in the Hemolymph of Manduca sexta Johannson (Lepidoptera: Sphingidae).
February 1974, Proceedings of the National Academy of Sciences of the United States of America,
Joshua K Young, and Anthony P Orth, and Walter G Goodman
Affinity purification and binding analysis of the hemolymph juvenile hormone binding protein from Manduca sexta.
August 1993, Biochemistry,
Joshua K Young, and Anthony P Orth, and Walter G Goodman
Effects of stress on the hemolymph juvenile hormone binding protein titers of Manduca sexta.
August 2007, Insect biochemistry and molecular biology,
Joshua K Young, and Anthony P Orth, and Walter G Goodman
Analysis and modification of thiols in the hemolymph juvenile hormone binding protein of Manduca sexta.
April 1993, Archives of biochemistry and biophysics,
Joshua K Young, and Anthony P Orth, and Walter G Goodman
Sequence, structure and expression of the hemolymph juvenile hormone binding protein gene in the tobacco hornworm, Manduca sexta.
January 2003, Insect biochemistry and molecular biology,
Joshua K Young, and Anthony P Orth, and Walter G Goodman
Cloning and sequencing of a cDNA for the hemolymph juvenile hormone binding protein of larval Manduca sexta.
November 1990, The Journal of biological chemistry,
Joshua K Young, and Anthony P Orth, and Walter G Goodman
The hemolymph titer of juvenile hormone binding protein and binding sites during the fourth larval instar of Manduca sexta.
May 1978, General and comparative endocrinology,
Joshua K Young, and Anthony P Orth, and Walter G Goodman
Purification and properties of the juvenile hormone carrier protein from the hemolymph of Manduca sexta.
January 1985, Methods in enzymology,
Joshua K Young, and Anthony P Orth, and Walter G Goodman
Binding specificity of the juvenile hormone carrier protein from the hemolymph of the tobacco hornworm Manduca sexta Johannson (Lepidoptera: Sphingidae).
May 1977, Biochemistry,
Copied contents to your clipboard!