Developmental pattern of calbindin D28k protein expression in the rat striatum and cerebral cortex. 2003

Barbara Litwinowicz, and Cezary Labuda, and Przemysław Kowiański, and Jan H Spodnik, and Beata Ludkiewicz, and Sławomir Wójcik, and Janusz Moryś
Department of Anatomy and Neurobiology, Medical University, Gdańsk, Poland. litwinowicz@konto.pl

We examined the protein expression of the calcium-binding protein calbindin D28k in two developing rat brain structures, the striatum and the cerebral cortex. The relative protein concentration level was quantified by means of the Western blotting method and densitometric scanning. 32 Wistar rats were used, divided according to survival period (P0-P120-postnatal days). Observations of the calbindin D28k protein expression in the rat striatum and the cerebral cortex revealed an increase in band color intensity between P0 and P10. The intensity of protein staining in older groups of animals stabilised at a similar level and in the P28 and P120 groups we observed a decrement of calbindin expression in the striatum. Calbindin D28k stabilises the intracellular calcium level, preventing calcium-induced apoptotic cell death in neurons. Thus, changes in calbindin D28k expression might be related to its neuroprotective role in differentiation and synaptogenesis during the postnatal development of the brain.

UI MeSH Term Description Entries
D002540 Cerebral Cortex The thin layer of GRAY MATTER on the surface of the CEREBRAL HEMISPHERES that develops from the TELENCEPHALON and folds into gyri and sulci. It reaches its highest development in humans and is responsible for intellectual faculties and higher mental functions. Allocortex,Archipallium,Cortex Cerebri,Cortical Plate,Paleocortex,Periallocortex,Allocortices,Archipalliums,Cerebral Cortices,Cortex Cerebrus,Cortex, Cerebral,Cortical Plates,Paleocortices,Periallocortices,Plate, Cortical
D003342 Corpus Striatum Striped GRAY MATTER and WHITE MATTER consisting of the NEOSTRIATUM and paleostriatum (GLOBUS PALLIDUS). It is located in front of and lateral to the THALAMUS in each cerebral hemisphere. The gray substance is made up of the CAUDATE NUCLEUS and the lentiform nucleus (the latter consisting of the GLOBUS PALLIDUS and PUTAMEN). The WHITE MATTER is the INTERNAL CAPSULE. Lenticular Nucleus,Lentiform Nucleus,Lentiform Nuclei,Nucleus Lentiformis,Lentiformis, Nucleus,Nuclei, Lentiform,Nucleus, Lenticular,Nucleus, Lentiform,Striatum, Corpus
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000831 Animals, Newborn Refers to animals in the period of time just after birth. Animals, Neonatal,Animal, Neonatal,Animal, Newborn,Neonatal Animal,Neonatal Animals,Newborn Animal,Newborn Animals
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D017208 Rats, Wistar A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain. Wistar Rat,Rat, Wistar,Wistar Rats
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D038081 Organogenesis Formation of differentiated cells and complicated tissue organization to provide specialized functions.
D064026 Calbindins Calcium-binding proteins that are found in DISTAL KIDNEY TUBULES, INTESTINES, BRAIN, and other tissues where they bind, buffer and transport cytoplasmic calcium. Calbindins possess a variable number of EF-HAND MOTIFS which contain calcium-binding sites. Some isoforms are regulated by VITAMIN D. Calbindin
D064030 S100 Calcium Binding Protein G A calbindin protein found in many mammalian tissues, including the UTERUS, PLACENTA, BONE, PITUITARY GLAND, and KIDNEYS. In intestinal ENTEROCYTES it mediates intracellular calcium transport from apical to basolateral membranes via calcium binding at two EF-HAND MOTIFS. Expression is regulated in some tissues by VITAMIN D. Calbindin 3,Calbindin D9K,Calbindin-D9K,Calcium Binding Protein, Vitamin D Dependent,Calcium-Binding Protein, Vitamin D-Dependent,Cholecalcin,IMCal Protein,Intestinal Membrane Calcium-Binding Protein,Vitamin D-Dependent Calcium-Binding Protein,Intestinal Membrane Calcium Binding Protein,Vitamin D Dependent Calcium Binding Protein

Related Publications

Barbara Litwinowicz, and Cezary Labuda, and Przemysław Kowiański, and Jan H Spodnik, and Beata Ludkiewicz, and Sławomir Wójcik, and Janusz Moryś
February 1992, The Journal of neuroscience : the official journal of the Society for Neuroscience,
Barbara Litwinowicz, and Cezary Labuda, and Przemysław Kowiański, and Jan H Spodnik, and Beata Ludkiewicz, and Sławomir Wójcik, and Janusz Moryś
July 1993, Anatomy and embryology,
Barbara Litwinowicz, and Cezary Labuda, and Przemysław Kowiański, and Jan H Spodnik, and Beata Ludkiewicz, and Sławomir Wójcik, and Janusz Moryś
December 1996, Neuroscience letters,
Barbara Litwinowicz, and Cezary Labuda, and Przemysław Kowiański, and Jan H Spodnik, and Beata Ludkiewicz, and Sławomir Wójcik, and Janusz Moryś
May 1993, Brain research,
Barbara Litwinowicz, and Cezary Labuda, and Przemysław Kowiański, and Jan H Spodnik, and Beata Ludkiewicz, and Sławomir Wójcik, and Janusz Moryś
June 1996, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research,
Barbara Litwinowicz, and Cezary Labuda, and Przemysław Kowiański, and Jan H Spodnik, and Beata Ludkiewicz, and Sławomir Wójcik, and Janusz Moryś
October 1993, Neuroreport,
Barbara Litwinowicz, and Cezary Labuda, and Przemysław Kowiański, and Jan H Spodnik, and Beata Ludkiewicz, and Sławomir Wójcik, and Janusz Moryś
October 1995, Anatomy and embryology,
Barbara Litwinowicz, and Cezary Labuda, and Przemysław Kowiański, and Jan H Spodnik, and Beata Ludkiewicz, and Sławomir Wójcik, and Janusz Moryś
February 2002, The journal of medical investigation : JMI,
Barbara Litwinowicz, and Cezary Labuda, and Przemysław Kowiański, and Jan H Spodnik, and Beata Ludkiewicz, and Sławomir Wójcik, and Janusz Moryś
March 2003, The European journal of neuroscience,
Barbara Litwinowicz, and Cezary Labuda, and Przemysław Kowiański, and Jan H Spodnik, and Beata Ludkiewicz, and Sławomir Wójcik, and Janusz Moryś
July 2014, Anatomical record (Hoboken, N.J. : 2007),
Copied contents to your clipboard!