Biomaterial Database

Dynamics of intestinal flora after oral vaccination with inactivated whole-cell/recombinant B subunit O139 cholera vaccine. 2004

Hui-xu Wu, and Qing Chen, and Jian-dong Li, and Xiu-fen Huang

Department of Epidemiology, First Military Medical University, Guangzhou 510515, China. qingchen@fimmu.com

OBJECTIVE To investigate the change of intestinal flora in subjects receiving oral vaccination with inactivated whole-cell/recombinant B subunit (WC/rBS) O139 cholera vaccine made in China. METHODS The fecal smears of 5 groups of subjects receiving the vaccine were collected before and 2 and 3 months after the vaccination to examine the distribution of the intestinal flora. RESULTS In the 458 specimens examined, the total amount of bacteria and percentages of G+b, G+c and G-c were decreased whereas the amount of G-b significantly increased after vaccination. Compared with the placebo control group, the vaccinated groups exhibited significant changes in G+b and G-c, which also varied significantly between the group receiving 3 doses of WC/rBS vaccine and the other vaccinated groups. The vaccination produced distinct changes in the percentage of the intestinal flora at different time points, showing the most obvious effect on G-b. Oral vaccination was shown to affect the percentage of the intestinal flora very likely through its effect on the amount of G-b. The percentages of G+b, G-b and G-c varied significantly between male and female subjects after vaccination. CONCLUSIONS Oral vaccination with WC/rBS affects the intestinal flora in human, and the mechanism as well as the implications needs to be further explored.

UI	MeSH Term	Description	Entries
D007422	Intestines	The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.	Intestine
D008297	Male		Males
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000284	Administration, Oral	The giving of drugs, chemicals, or other substances by mouth.	Drug Administration, Oral,Administration, Oral Drug,Oral Administration,Oral Drug Administration,Administrations, Oral,Administrations, Oral Drug,Drug Administrations, Oral,Oral Administrations,Oral Drug Administrations
D000293	Adolescent	A person 13 to 18 years of age.	Adolescence,Youth,Adolescents,Adolescents, Female,Adolescents, Male,Teenagers,Teens,Adolescent, Female,Adolescent, Male,Female Adolescent,Female Adolescents,Male Adolescent,Male Adolescents,Teen,Teenager,Youths
D014611	Vaccination	Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.	Immunization, Active,Active Immunization,Active Immunizations,Immunizations, Active,Vaccinations
D014614	Vaccines, Synthetic	Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. The latter vaccines may also be whole viruses whose nucleic acids have been modified.	Antigens, Synthetic,Chemical Vaccine,Chemical Vaccines,Immunogens, Synthetic,Molecular Vaccine,Molecular Vaccines,Recombinant Vaccine,Semisynthetic Vaccine,Semisynthetic Vaccines,Synthetic Antigen,Synthetic Vaccine,Synthetic Vaccines,Vaccines, Recombinant,Synthetic Antigens,Synthetic Immunogens,Vaccines, Chemical,Vaccines, Molecular,Vaccines, Semisynthetic,Antigen, Synthetic,Recombinant Vaccines,Vaccine, Chemical,Vaccine, Molecular,Vaccine, Recombinant,Vaccine, Semisynthetic,Vaccine, Synthetic
D015164	Vaccines, Inactivated	Vaccines in which the infectious microbial nucleic acid components have been destroyed by chemical or physical treatment (e.g., formalin, beta-propiolactone, gamma radiation) without affecting the antigenicity or immunogenicity of the viral coat or bacterial outer membrane proteins.	Inactivated Vaccine,Killed Vaccine,Killed Vaccines,Vaccines, Killed,Inactivated Vaccines,Vaccine, Inactivated,Vaccine, Killed
D022121	Cholera Vaccines	Vaccines or candidate vaccines used to prevent infection with VIBRIO CHOLERAE. The original cholera vaccine consisted of killed bacteria, but other kinds of vaccines now exist.	Cholera Vaccine,Vaccine, Cholera,Vaccines, Cholera