BIOMAT Database Logo BIOMAT Database Logo

Immortalization of human osteoblasts by transferring human telomerase reverse transcriptase gene. 2004

Yin Xiaoxue, and Chen Zhongqiang, and Guo Zhaoqing, and Dang Gengting, and Ma Qingjun, and Wang Shenwu
Department of Orthopaedics, Peking University Third Hospital, Haidian District, Beijing 100083, China. yxx2733_cn@sina.com

In the current study, in order to establish an immortalized osteoblast cell line, human mesenchymal stem cells (hMSCs) had been inducted into osteoblasts directionally by an osteo-inductive conditioned medium, then the osteoblasts were steadily transduced by a retroviral vector containing human telomerase reverse transcriptase (hTERT) gene. The expression of hTERT, the telomerase activity, the telomere lengths, the tumorigenesis and the osteogenesis characteristics of transduced cells at different population doublings (PDs) and the primary normal human osteoblast (hOB) were identified. The results demonstrated that hTERT gene had been transferred into human osteoblasts successfully; the transduced cell line-clone5 expressed telomerase activity and divided vigorously and now have undergone more than 120 PDs; The telomere length of clone5 elongated and was stable; Different eras of clone5 (PD 40 and PD 88) both expressed bone-specific markers, such as alkaline phosphatase (ALP), collagen type I, and osteopontin. And the quantitative assay of ALP activity showed that there were no significant differences among untransduced cells, PD 40 and PD 88 clone5 cells. Furthermore, the immortalized cell line was benign in nude mice tumor formation and soft agar colony formation assay.

UI MeSH Term Description Entries
D008648 Mesoderm The middle germ layer of an embryo derived from three paired mesenchymal aggregates along the neural tube. Mesenchyme,Dorsal Mesoderm,Intermediate Mesoderm,Lateral Plate Mesoderm,Mesenchyma,Paraxial Mesoderm,Dorsal Mesoderms,Intermediate Mesoderms,Lateral Plate Mesoderms,Mesenchymas,Mesoderm, Dorsal,Mesoderm, Intermediate,Mesoderm, Lateral Plate,Mesoderm, Paraxial,Mesoderms, Dorsal,Mesoderms, Intermediate,Mesoderms, Lateral Plate,Mesoderms, Paraxial,Paraxial Mesoderms,Plate Mesoderm, Lateral,Plate Mesoderms, Lateral
D010006 Osteoblasts Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone. Osteoblast
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002999 Clone Cells A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed) Clones,Cell, Clone,Cells, Clone,Clone,Clone Cell
D003593 Cytoplasm The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990) Protoplasm,Cytoplasms,Protoplasms
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins
D005822 Genetic Vectors DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition. Cloning Vectors,Shuttle Vectors,Vectors, Genetic,Cloning Vector,Genetic Vector,Shuttle Vector,Vector, Cloning,Vector, Genetic,Vector, Shuttle,Vectors, Cloning,Vectors, Shuttle
D006367 HeLa Cells The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays. Cell, HeLa,Cells, HeLa,HeLa Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000256 Adenoviridae A family of non-enveloped viruses infecting mammals (MASTADENOVIRUS) and birds (AVIADENOVIRUS) or both (ATADENOVIRUS). Infections may be asymptomatic or result in a variety of diseases. Adenoviruses,Ichtadenovirus,Adenovirus,Ichtadenoviruses

Related Publications

Yin Xiaoxue, and Chen Zhongqiang, and Guo Zhaoqing, and Dang Gengting, and Ma Qingjun, and Wang Shenwu
Immortalization of bovine mammary epithelial cells alone by human telomerase reverse transcriptase.
April 2010, Cell biology international,
Yin Xiaoxue, and Chen Zhongqiang, and Guo Zhaoqing, and Dang Gengting, and Ma Qingjun, and Wang Shenwu
Immortalization of human CD8+ T cell clones by ectopic expression of telomerase reverse transcriptase.
October 2000, Journal of immunology (Baltimore, Md. : 1950),
Yin Xiaoxue, and Chen Zhongqiang, and Guo Zhaoqing, and Dang Gengting, and Ma Qingjun, and Wang Shenwu
Immortalization of swine umbilical vein endothelial cells with human telomerase reverse transcriptase.
December 2007, Molecules and cells,
Yin Xiaoxue, and Chen Zhongqiang, and Guo Zhaoqing, and Dang Gengting, and Ma Qingjun, and Wang Shenwu
Differential gene expressions during immortalization of normal human fibroblasts and endothelial cells transfected with human telomerase reverse transcriptase gene.
June 2004, International journal of oncology,
Yin Xiaoxue, and Chen Zhongqiang, and Guo Zhaoqing, and Dang Gengting, and Ma Qingjun, and Wang Shenwu
Telomerase Reverse Transcriptase Increases Proliferation and Lifespan of Human NK Cells without Immortalization.
June 2021, Biomedicines,
Yin Xiaoxue, and Chen Zhongqiang, and Guo Zhaoqing, and Dang Gengting, and Ma Qingjun, and Wang Shenwu
Regulation of the human telomerase reverse transcriptase gene.
January 2002, Oncogene,
Yin Xiaoxue, and Chen Zhongqiang, and Guo Zhaoqing, and Dang Gengting, and Ma Qingjun, and Wang Shenwu
Immortalization of mesenchymal stem cells from bone marrow of rhesus monkey by transfection with human telomerase reverse transcriptase gene.
March 2008, Transplantation proceedings,
Yin Xiaoxue, and Chen Zhongqiang, and Guo Zhaoqing, and Dang Gengting, and Ma Qingjun, and Wang Shenwu
Reversible immortalization of sheep fetal fibroblast cells by tetracycline-inducible expression of human telomerase reverse transcriptase.
August 2016, Biotechnology letters,
Yin Xiaoxue, and Chen Zhongqiang, and Guo Zhaoqing, and Dang Gengting, and Ma Qingjun, and Wang Shenwu
[Immortalization of human osteoblast by transferring hTERT gene].
July 2003, Zhonghua yi xue za zhi,
Yin Xiaoxue, and Chen Zhongqiang, and Guo Zhaoqing, and Dang Gengting, and Ma Qingjun, and Wang Shenwu
Upregulation of survivin during immortalization of nontransformed human fibroblasts transduced with telomerase reverse transcriptase.
July 2009, Oncogene,
Copied contents to your clipboard!