Salmonella enterica subsp. enterica serovar Enteritidis harbours ColE1, ColE2, and rolling-circle-like replicating plasmids. 2004

Daniela Gregorova, and Jitka Matiasovicova, and Alena Sebkova, and Marcela Faldynova, and Ivan Rychlik
Veterinary Research Institute, Hudcova 70, 621-32 Brno, Czech Republic.

Using DNA hybridization, at least three distinct groups of low molecular mass plasmids were identified in Salmonella enterica subsp. enterica serovar Enteritidis. After sequencing representative plasmids from each group, we concluded that they belonged to ColE1, ColE2, and rolling-circle-like replicating plasmids. Plasmid pK (4245 bp) is a representative of widely distributed ColE1 plasmids. Plasmid pP (4301 bp) is homologous to ColE2 plasmids and was present predominantly in single-stranded DNA form. The smallest plasmids pJ (2096 bp) and pB (1983 bp) were classified as rolling-circle-like replicating plasmids. Both encoded only a single protein essential for their own replication, and they must have existed in an unusual molecular structure, as (i) they were capable of hybridization without denaturation, (ii) their DNA could be linearized with S1 nuclease, and (iii) even after such treatment, the ability to hybridize without denaturation did not disappear.

UI MeSH Term Description Entries
D009691 Nucleic Acid Denaturation Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible. DNA Denaturation,DNA Melting,RNA Denaturation,Acid Denaturation, Nucleic,Denaturation, DNA,Denaturation, Nucleic Acid,Denaturation, RNA,Nucleic Acid Denaturations
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D003086 Bacteriocin Plasmids Plasmids encoding bacterial exotoxins (BACTERIOCINS). Bacteriocin Factors,Col Factors,Colicin Factors,Colicin Plasmids,Bacteriocin Factor,Bacteriocin Plasmid,Col Factor,Colicin Factor,Colicin Plasmid,Factor, Bacteriocin,Factor, Col,Factor, Colicin,Factors, Bacteriocin,Factors, Col,Factors, Colicin,Plasmid, Bacteriocin,Plasmid, Colicin,Plasmids, Bacteriocin,Plasmids, Colicin
D004261 DNA Replication The process by which a DNA molecule is duplicated. Autonomous Replication,Replication, Autonomous,Autonomous Replications,DNA Replications,Replication, DNA,Replications, Autonomous,Replications, DNA
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D004277 DNA, Single-Stranded A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle. Single-Stranded DNA,DNA, Single Stranded,Single Stranded DNA
D005111 Extrachromosomal Inheritance Vertical transmission of hereditary characters by DNA from cytoplasmic organelles such as MITOCHONDRIA; CHLOROPLASTS; and PLASTIDS, or from PLASMIDS or viral episomal DNA. Cytoplasmic Inheritance,Extranuclear Inheritance,Inheritance, Cytoplasmic,Inheritance, Extrachromosomal,Inheritance, Extranuclear
D012477 Salmonella enteritidis A serotype of Salmonella enterica which is an etiologic agent of gastroenteritis in man and other animals. Salmonella enterica serovar enteritidis
D015719 Single-Strand Specific DNA and RNA Endonucleases Enzymes that catalyze the endonucleolytic cleavage of single-stranded regions of DNA or RNA molecules while leaving the double-stranded regions intact. They are particularly useful in the laboratory for producing "blunt-ended" DNA molecules from DNA with single-stranded ends and for sensitive GENETIC TECHNIQUES such as NUCLEASE PROTECTION ASSAYS that involve the detection of single-stranded DNA and RNA. Single Strand Specific DNA and RNA Endonucleases

Related Publications

Daniela Gregorova, and Jitka Matiasovicova, and Alena Sebkova, and Marcela Faldynova, and Ivan Rychlik
January 1996, Journal of medical microbiology,
Daniela Gregorova, and Jitka Matiasovicova, and Alena Sebkova, and Marcela Faldynova, and Ivan Rychlik
April 1997, Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases,
Daniela Gregorova, and Jitka Matiasovicova, and Alena Sebkova, and Marcela Faldynova, and Ivan Rychlik
May 2010, Plasmid,
Daniela Gregorova, and Jitka Matiasovicova, and Alena Sebkova, and Marcela Faldynova, and Ivan Rychlik
October 2014, Genome announcements,
Daniela Gregorova, and Jitka Matiasovicova, and Alena Sebkova, and Marcela Faldynova, and Ivan Rychlik
January 2011, Acta crystallographica. Section F, Structural biology and crystallization communications,
Daniela Gregorova, and Jitka Matiasovicova, and Alena Sebkova, and Marcela Faldynova, and Ivan Rychlik
January 2009, Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology],
Daniela Gregorova, and Jitka Matiasovicova, and Alena Sebkova, and Marcela Faldynova, and Ivan Rychlik
March 2007, Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin,
Daniela Gregorova, and Jitka Matiasovicova, and Alena Sebkova, and Marcela Faldynova, and Ivan Rychlik
December 2003, Emerging infectious diseases,
Daniela Gregorova, and Jitka Matiasovicova, and Alena Sebkova, and Marcela Faldynova, and Ivan Rychlik
April 2014, Genome announcements,
Daniela Gregorova, and Jitka Matiasovicova, and Alena Sebkova, and Marcela Faldynova, and Ivan Rychlik
November 2015, Italian journal of food safety,
Copied contents to your clipboard!