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Using different conditions for incubation and fluorometry with 4-methylumbelliferylglycosides as substrates, we demonstrated the presence of acid alpha-glucosidase, "renal" alpha-glucosidase, N-acetyl-beta-D-glucosaminidase A, and N-acetyl-beta-D-glucosaminidase B in freshly drawn normal human serum. The acid alpha-glucosidase enzymatic activity was determined at pH 4.0 in 0.1 mol/L Tris reagent, whereas the renal isoenzyme activity was determined at pH 5.6 in presence of 0.05 mol/L turanose reagent. N-Acetyl-beta-D-glucosaminidases A and B were determined by their different behaviors on heating. The corresponding reference intervals for each enzyme were calculated from results for 40 controls: acid alpha-glucosidase (0.024 +/- 0.010 U/L), renal alpha-glucosidase (0.035 +/- 0.012 U/L), N-acetyl-beta-D-glucosaminidase A (10.2 +/- 2.9 U/L), and N-acetyl-beta-D-glucosaminidase B (4.4 +/- 2.1 U/L).
P Coma, and
L Gomez-Chacon, and
B Garcia-Serrano, and
E Fernandez, and
M A Ortiz-Apodaca
July 1988,
Minerva medica,
P Coma, and
L Gomez-Chacon, and
B Garcia-Serrano, and
E Fernandez, and
M A Ortiz-Apodaca
August 1984,
Clinica chimica acta; international journal of clinical chemistry,
P Coma, and
L Gomez-Chacon, and
B Garcia-Serrano, and
E Fernandez, and
M A Ortiz-Apodaca
September 1986,
Clinica chimica acta; international journal of clinical chemistry,
P Coma, and
L Gomez-Chacon, and
B Garcia-Serrano, and
E Fernandez, and
M A Ortiz-Apodaca
January 1976,
Human genetics,
P Coma, and
L Gomez-Chacon, and
B Garcia-Serrano, and
E Fernandez, and
M A Ortiz-Apodaca
March 1992,
JPMA. The Journal of the Pakistan Medical Association,
P Coma, and
L Gomez-Chacon, and
B Garcia-Serrano, and
E Fernandez, and
M A Ortiz-Apodaca
May 1974,
Zeitschrift fur klinische Chemie und klinische Biochemie,
P Coma, and
L Gomez-Chacon, and
B Garcia-Serrano, and
E Fernandez, and
M A Ortiz-Apodaca
May 1995,
Nihon rinsho. Japanese journal of clinical medicine,
P Coma, and
L Gomez-Chacon, and
B Garcia-Serrano, and
E Fernandez, and
M A Ortiz-Apodaca
January 1998,
Nihon rinsho. Japanese journal of clinical medicine,
P Coma, and
L Gomez-Chacon, and
B Garcia-Serrano, and
E Fernandez, and
M A Ortiz-Apodaca
August 1999,
Nihon rinsho. Japanese journal of clinical medicine,
P Coma, and
L Gomez-Chacon, and
B Garcia-Serrano, and
E Fernandez, and
M A Ortiz-Apodaca
January 1994,
Romanian journal of physiology : physiological sciences,
