The toxic effects of nonylphenol on the reproductive system of male rats. 2004

Xiao D Han, and Zhi G Tu, and Yi Gong, and Su N Shen, and Xu Y Wang, and Li N Kang, and Ya Y Hou, and Jian X Chen
Immunology and Reproductive Biology Laboratory, Medical School, Nanjing University, Nanjing, Jiangsu 210093, PR China. hanxdnju@hotmail.com

Male Sprague-Dawley (SD) rats were exposed to 4-t-nonylphenol (NP) by gavage at dosages of 0, 125 and 250 mg/kg/day for 50 days. Organ weights of liver, kidney, testis and epididymis were measured. Sperm number in the head of epididymis was counted. Several hormones including testosterone, luteinizing hormone and follicle stimulating hormone were measured. Testicular sections were observed by light and electron microscopy. Terminal dideoxynucleotidyl transferase dUTP nick end labeling assay was performed to probe the apoptotic cells in seminiferous tubules. When rats were treated with nonylphenol at 250 mg/kg/day, the absolute and relative weight of epididymis decreased dramatically, while the relative weights of kidney and liver increased by 14 and 22%, respectively. In addition, the sperm density of the head of epididymis and the testosterone level descended at 250 mg/kg/day. The levels of luteinizing hormone and follicle stimulating hormone increased in both nonylphenol treated groups. Pathological changes were detected by microscopy and the transferase dUTP nick end labeling (TUNEL) assay showed that the number of apoptotic cells in testes increased with nonylphenol in a dose-dependent manner.

UI MeSH Term Description Entries
D007668 Kidney Body organ that filters blood for the secretion of URINE and that regulates ion concentrations. Kidneys
D007986 Luteinizing Hormone A major gonadotropin secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Luteinizing hormone regulates steroid production by the interstitial cells of the TESTIS and the OVARY. The preovulatory LUTEINIZING HORMONE surge in females induces OVULATION, and subsequent LUTEINIZATION of the follicle. LUTEINIZING HORMONE consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the three pituitary glycoprotein hormones (TSH, LH and FSH), but the beta subunit is unique and confers its biological specificity. ICSH (Interstitial Cell Stimulating Hormone),Interstitial Cell-Stimulating Hormone,LH (Luteinizing Hormone),Lutropin,Luteoziman,Luteozyman,Hormone, Interstitial Cell-Stimulating,Hormone, Luteinizing,Interstitial Cell Stimulating Hormone
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008297 Male Males
D009929 Organ Size The measurement of an organ in volume, mass, or heaviness. Organ Volume,Organ Weight,Size, Organ,Weight, Organ
D010636 Phenols Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D004822 Epididymis The convoluted cordlike structure attached to the posterior of the TESTIS. Epididymis consists of the head (caput), the body (corpus), and the tail (cauda). A network of ducts leaving the testis joins into a common epididymal tubule proper which provides the transport, storage, and maturation of SPERMATOZOA.
D005640 Follicle Stimulating Hormone A major gonadotropin secreted by the adenohypophysis (PITUITARY GLAND, ANTERIOR). Follicle-stimulating hormone stimulates GAMETOGENESIS and the supporting cells such as the ovarian GRANULOSA CELLS, the testicular SERTOLI CELLS, and LEYDIG CELLS. FSH consists of two noncovalently linked subunits, alpha and beta. Within a species, the alpha subunit is common in the three pituitary glycoprotein hormones (TSH, LH, and FSH), but the beta subunit is unique and confers its biological specificity. FSH (Follicle Stimulating Hormone),Follicle-Stimulating Hormone,Follitropin
D000284 Administration, Oral The giving of drugs, chemicals, or other substances by mouth. Drug Administration, Oral,Administration, Oral Drug,Oral Administration,Oral Drug Administration,Administrations, Oral,Administrations, Oral Drug,Drug Administrations, Oral,Oral Administrations,Oral Drug Administrations

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