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The structure of the Gln25 variant of ribonuclease T1 (RNase T1) crystallized at pH 7 and at high ionic strength has been solved by molecular replacement using the coordinates of the Lys25-RNase T1/2'-guanylic acid (2'GMP) complex at pH 5 [Arni et al. (1988) J. Biol. Chem. 263, 15358-15368] and refined by energy minimization and stereochemically restrained least-squares minimization to a crystallographic R-factor of 14.4% at 1.84-A resolution. The asymmetric unit contains three molecules, and the final model consists of 2302 protein atoms, 3 sulfates (at the catalytic sites), and 179 solvent water molecules. The estimated root mean square (rms) error in the coordinates is 0.15 A, and the rms deviation from ideality is 0.018 A for bond lengths and 1.8 degrees for bond angles. Significant differences are observed between the three molecules in the asymmetric unit at the base recognition and catalytic sites.
R K Arni, and
G P Pal, and
K G Ravichandran, and
A Tulinsky, and
F G Walz, and
P Metcalf
August 2003,
Biological chemistry,
R K Arni, and
G P Pal, and
K G Ravichandran, and
A Tulinsky, and
F G Walz, and
P Metcalf
November 1991,
Journal of molecular biology,
R K Arni, and
G P Pal, and
K G Ravichandran, and
A Tulinsky, and
F G Walz, and
P Metcalf
October 1988,
The Journal of biological chemistry,
R K Arni, and
G P Pal, and
K G Ravichandran, and
A Tulinsky, and
F G Walz, and
P Metcalf
April 1985,
FEBS letters,
R K Arni, and
G P Pal, and
K G Ravichandran, and
A Tulinsky, and
F G Walz, and
P Metcalf
April 1989,
Journal of molecular biology,
R K Arni, and
G P Pal, and
K G Ravichandran, and
A Tulinsky, and
F G Walz, and
P Metcalf
October 1983,
FEBS letters,
R K Arni, and
G P Pal, and
K G Ravichandran, and
A Tulinsky, and
F G Walz, and
P Metcalf
January 1993,
European journal of biochemistry,
R K Arni, and
G P Pal, and
K G Ravichandran, and
A Tulinsky, and
F G Walz, and
P Metcalf
June 1991,
FEBS letters,
R K Arni, and
G P Pal, and
K G Ravichandran, and
A Tulinsky, and
F G Walz, and
P Metcalf
October 1995,
Journal of biochemistry,
R K Arni, and
G P Pal, and
K G Ravichandran, and
A Tulinsky, and
F G Walz, and
P Metcalf
August 1991,
The Journal of biological chemistry,
