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[Detection of flaviviruses by reverse-transcription polymerase chain reaction with universal primer set]. 1997

M Fang, and C Chen, and H Chen, and X Tian, and L Jiang, and Y Rao, and H Guo
Military Medical Institute, Guangzhou Military District, Department of Microbiology, Sun Yat-sen University of Medical Sciences, Guangzhou 510507.

For the purpose of early and rapid diagnosis of flavivirus infection, the universal primer set was selected on the NS1 gene. Another five different internal primers were selected on the NS1 genes of DEN1, 2, 3, 4 and JEV. With the universal primer set designed, the NS1 fragment about the size of 413 bp and with the five internal primers, the NS1 fragments about the sizes of 262 bp (DEN1), 189 bp (DEN2), 392 bp (DEN3), 97 bp (DEN4) and 323 bp (JEV) were amplified respectively. The reverse transcription-polymerase chain reaction (RT-PCR) was used to amplify gene fragments of flaviviruses. cDNA of DEN1, 2, 3, 4 and JEV were successfully amplified with universal primer set and internal primers. Sera from 78 patients with dengue fever were assayed by nested PCR, DEN1 was detected from 18 of the 40 patients' sera and DEN2 was detected from 48 patients' sera. Sera from 42 patients with Japanese encephalitis were also assayed by nested PCR, JEV was detected from 35 of the 42 patients' sera. By nested PCR, we completed identification of flaviviruses within 2 days. The results showed that this method has the potential value in rapid clinical diagnosis of flavivirus infection.

UI MeSH Term Description Entries
D003715 Dengue An acute febrile disease transmitted by the bite of AEDES mosquitoes infected with DENGUE VIRUS. It is self-limiting and characterized by fever, myalgia, headache, and rash. SEVERE DENGUE is a more virulent form of dengue. Dengue Fever,Break-Bone Fever,Breakbone Fever,Classical Dengue,Classical Dengue Fever,Break Bone Fever,Classical Dengue Fevers,Classical Dengues,Dengue Fever, Classical,Dengue, Classical,Fever, Break-Bone,Fever, Breakbone,Fever, Dengue
D003716 Dengue Virus A species of the genus FLAVIVIRUS which causes an acute febrile and sometimes hemorrhagic disease in man. Dengue is mosquito-borne and four serotypes are known. Breakbone Fever Virus,Breakbone Fever Viruses,Dengue Viruses,Fever Virus, Breakbone,Fever Viruses, Breakbone,Virus, Breakbone Fever,Virus, Dengue,Viruses, Breakbone Fever,Viruses, Dengue
D005416 Flavivirus A genus of FLAVIVIRIDAE containing several subgroups and many species. Most are arboviruses transmitted by mosquitoes or ticks. The type species is YELLOW FEVER VIRUS. Arbovirus, Group B,Arboviruses, Group B,Flaviviruses,Group B Arbovirus,Group B Arboviruses
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D017361 Viral Nonstructural Proteins Proteins encoded by a VIRAL GENOME that are not structural components of VIRUS PARTICLES. Some of these proteins may play roles within the infected cell during VIRUS REPLICATION or act in regulation of virus replication or VIRUS ASSEMBLY. Nonstructural Proteins, Viral,NS Proteins, Viral,Viral NS Proteins,Viral Non-Structural Proteins,Viral Nonstructural Protein,Viral Nonstructural Proteins NS1,Viral Nonstructural Proteins NS2,Nonstructural Protein, Viral,Viral Non Structural Proteins
D017931 DNA Primers Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques. DNA Primer,Oligodeoxyribonucleotide Primer,Oligodeoxyribonucleotide Primers,Oligonucleotide Primer,Oligonucleotide Primers,Primer, DNA,Primer, Oligodeoxyribonucleotide,Primer, Oligonucleotide,Primers, DNA,Primers, Oligodeoxyribonucleotide,Primers, Oligonucleotide
D018177 Flavivirus Infections Infections with viruses of the genus FLAVIVIRUS, family FLAVIVIRIDAE. Infections, Flavivirus,Flavivirus Infection,Infection, Flavivirus
D020133 Reverse Transcriptase Polymerase Chain Reaction A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols. Polymerase Chain Reaction, Reverse Transcriptase,Reverse Transcriptase PCR,PCR, Reverse Transcriptase,Transcriptase PCR, Reverse

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