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Using a universal primer set designed to match the sequence of the NS1 gene of flaviviruses, the virus RNA of dengue (DEN), Japanese encephalitis (JEV), powassan and langat of Flaviviridae were successfully amplified by polymerase chain reaction (PCR) via cDNA; and with different internal primers, the serotypes of the dengue viruses were identified. Of the 78 clinically diagnosed dengue fever patients, 18 patients were positive for DEN 1, 48 patients for DEN 2 and 8 patients concurrently infected with DEN 4. Of the 52 patients admitted with Japanese encephalitis (JE), 45 were determined to be JEV infections. By nested PCR, we completed the identification of flaviviruses within 2 days. The results show that seven primers have a potential value for rapid clinical diagnosis of flavivirus infections.
F Meiyu, and
C Huosheng, and
C Cuihua, and
T Xiaodong, and
J Lianhua, and
P Yifei, and
C Weijun, and
G Huiyu
September 1997,
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology,
F Meiyu, and
C Huosheng, and
C Cuihua, and
T Xiaodong, and
J Lianhua, and
P Yifei, and
C Weijun, and
G Huiyu
April 1991,
Journal of medical virology,
F Meiyu, and
C Huosheng, and
C Cuihua, and
T Xiaodong, and
J Lianhua, and
P Yifei, and
C Weijun, and
G Huiyu
May 2006,
Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases,
F Meiyu, and
C Huosheng, and
C Cuihua, and
T Xiaodong, and
J Lianhua, and
P Yifei, and
C Weijun, and
G Huiyu
August 2009,
Avian pathology : journal of the W.V.P.A,
F Meiyu, and
C Huosheng, and
C Cuihua, and
T Xiaodong, and
J Lianhua, and
P Yifei, and
C Weijun, and
G Huiyu
September 2000,
Journal of clinical oncology : official journal of the American Society of Clinical Oncology,
F Meiyu, and
C Huosheng, and
C Cuihua, and
T Xiaodong, and
J Lianhua, and
P Yifei, and
C Weijun, and
G Huiyu
April 1996,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
F Meiyu, and
C Huosheng, and
C Cuihua, and
T Xiaodong, and
J Lianhua, and
P Yifei, and
C Weijun, and
G Huiyu
January 2004,
Veterinaria italiana,
F Meiyu, and
C Huosheng, and
C Cuihua, and
T Xiaodong, and
J Lianhua, and
P Yifei, and
C Weijun, and
G Huiyu
May 1997,
Chinese medical journal,
F Meiyu, and
C Huosheng, and
C Cuihua, and
T Xiaodong, and
J Lianhua, and
P Yifei, and
C Weijun, and
G Huiyu
January 1998,
Methods in molecular medicine,
F Meiyu, and
C Huosheng, and
C Cuihua, and
T Xiaodong, and
J Lianhua, and
P Yifei, and
C Weijun, and
G Huiyu
January 2001,
Methods in molecular medicine,
