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A biosynthesis activity of Streptomyces recifensis var. lyticus. 2004

I E Sokolova, and T P Kylochek, and A I Vinnikov
Dniepropetrovsk State University, Dniepropetrovsk, Ukraine.

The structure of enzyme complex synthesized of soil streptomycete was investigated. Fractionation and purification of enzymes have allowed to identify a line of hydrolases: proteinases, amylases, DNA-ses, cellulases and also glycosidases and endopeptidases with lytic action. Their properties were studied. The isolated lytic endopeptidases had a wide substrate specificity and hydrolyzed casein and synthetic peptides at pH 7-9 and also a cell wall of bacteria at pH 5-6. The temperature optimum of 5 researched endopeptidases are in the range 45-60 degrees C. They were activated by ions Co2+, Fe2+, Cu2+ to a different degree. The universal activators of lytic endopeptidases were ions Cd2+ and Pb2+. They have raised lytic activity of enzymes 3-7 times. The obtained data can witness high adaptogenic ability of streptomycetes in relation to heavy metals. The possibility of enzyme preparation from Streptomyces recifensis use for growth stimulation of agricultural plants was shown also against the wheat, oats, maize, haricot beans, peas, barley.

UI MeSH Term Description Entries
D010447 Peptide Hydrolases Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES. Peptidase,Peptidases,Peptide Hydrolase,Protease,Proteases,Proteinase,Proteinases,Proteolytic Enzyme,Proteolytic Enzymes,Esteroproteases,Enzyme, Proteolytic,Hydrolase, Peptide
D001433 Bacteriolysis Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES. Bacteriolyses
D012988 Soil Microbiology The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms. Microbiology, Soil
D013302 Streptomyces A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.
D013379 Substrate Specificity A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts. Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D014644 Genetic Variation Genotypic differences observed among individuals in a population. Genetic Diversity,Variation, Genetic,Diversity, Genetic,Diversities, Genetic,Genetic Diversities,Genetic Variations,Variations, Genetic

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