Biomaterial Database

[The use of a protoplast-generating method in selecting the lytic enzyme producer Streptomyces recifensis subsp. lyticus]. 1994

T Ia Kulikova, and A S Sten'ko, and N K Bezkorovaĭnaia, and Iu S Babenko

Optimal conditions for the formation of stable protoplasts of S. recifensis subsp. lyticus 2435 were defined and the use of the protoplasting procedure to increase the activity of the synthesis of extracellular enzymes was shown in principle possible. The level of the synthesis of glycosidases and lytic endopeptidases by the isolated variant II-29 was higher than that the initial strain by 40-45 per cent which was due to the enrichment of the population with more active variants.

UI	MeSH Term	Description	Entries
D008837	Micrococcus	A genus of gram-positive, spherical bacteria found in soils and fresh water, and frequently on the skin of man and other animals.
D010447	Peptide Hydrolases	Hydrolases that specifically cleave the peptide bonds found in PROTEINS and PEPTIDES. Examples of sub-subclasses for this group include EXOPEPTIDASES and ENDOPEPTIDASES.	Peptidase,Peptidases,Peptide Hydrolase,Protease,Proteases,Proteinase,Proteinases,Proteolytic Enzyme,Proteolytic Enzymes,Esteroproteases,Enzyme, Proteolytic,Hydrolase, Peptide
D011523	Protoplasts	The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL.	Protoplast
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D001412	Bacillus subtilis	A species of gram-positive bacteria that is a common soil and water saprophyte.	Natto Bacteria,Bacillus subtilis (natto),Bacillus subtilis subsp. natto,Bacillus subtilis var. natto
D001431	Bacteriological Techniques	Techniques used in studying bacteria.	Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic
D012641	Selection, Genetic	Differential and non-random reproduction of different genotypes, operating to alter the gene frequencies within a population.	Natural Selection,Genetic Selection,Selection, Natural
D013211	Staphylococcus aureus	Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
D013302	Streptomyces	A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.