Biomaterial Database

Water-soluble complexes through coulombic interactions between bovine serum albumin and anionic polyelectrolytes grafted with hydrophilic nonionic side chains. 2005

Maria Sotiropoulou, and Georgios Bokias, and Georgios Staikos

Department of Chemical Engineering, University of Patras, GR-265 04 Patras, Greece.

The interaction between bovine serum albumin (BSA) and the anionic graft copolymers poly(sodium acrylate-co-sodium 2-acrylamido-2-methyl-1-propanesulfonate)-graft-poly(N,N-dimethylacrylamide) (P(NaA-co-NaAMPS)-g-PDMAMx) was investigated within the acid pH region, 2 < or = pH < or = 7. The weight percentage, x, of the poly(N,N-dimethylacrylamide) (PDMAM) side chains varied from 0 up to 75% (w:w). When BSA and P(NaA-co-NaAMPS)-g-PDMAMx are oppositely charged, i.e., when pH is lower than the isoelectric point of BSA, the two macromolecules associate through Coulombic attractions. When the anionic graft copolymer is rich enough to the nonionic PDMAM side chains, x > or = 50% w:w, the associative phase separation is practically prevented, as revealed by the turbidimetric study of the BSA/P(NaA-co-NaAMPS)-g-PDMAMx mixtures in aqueous solution vs pH. In addition, the viscosity measurements support the formation through a charge neutralization process of a rather compact protein-polyelectrolyte complex stabilized by the hydrophilic PDMAM side chains grafted onto the anionic copolymer backbone.

UI	MeSH Term	Description	Entries
D009391	Nephelometry and Turbidimetry	Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering. In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam.	Turbidimetry,Nephelometry,Turbidimetry and Nephelometry
D004573	Electrolytes	Substances that dissociate into two or more ions, to some extent, in water. Solutions of electrolytes thus conduct an electric current and can be decomposed by it (ELECTROLYSIS). (Grant & Hackh's Chemical Dictionary, 5th ed)	Electrolyte
D000838	Anions	Negatively charged atoms, radicals or groups of atoms which travel to the anode or positive pole during electrolysis.	Anion
D012710	Serum Albumin, Bovine	Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)	Fetal Bovine Serum,Fetal Calf Serum,Albumin Bovine,Bovine Albumin,Bovine Serum Albumin,Albumin, Bovine,Albumin, Bovine Serum,Bovine Serum, Fetal,Bovine, Albumin,Calf Serum, Fetal,Serum, Fetal Bovine,Serum, Fetal Calf
D012995	Solubility	The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Solubilities
D014783	Viscosity	The resistance that a gaseous or liquid system offers to flow when it is subjected to shear stress. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Viscosities
D014867	Water	A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Hydrogen Oxide
D055672	Static Electricity	The accumulation of an electric charge on a object	Electrostatic,Electrostatics,Static Charge,Charge, Static,Charges, Static,Electricity, Static,Static Charges