Biomaterial Database

[Soluble complexes of bovine serum albumin with linear polyelectrolytes]. 1978

V A Kabanov, and M I Mustafaev, and V V Belova, and V P Evdakov

Interaction of bonive serum albumin with copolymers 2-methyl-5-vinylpyridine, acrylic and methacrylic acids in different proportions in aqueous solutions was studied. It was shown, that in a wide range of ration of the components soluble cooperative complexes were formed. Ability of amphotheric copolymers to form complexes with bovine serum albumin depend both on the composition of the copolymers and on the pH of the medium. The non-polar interactions of the protein globules with hydrocarbonic 2-methyl-5-vinylpyridine chain fragments of the polyampholyte play an important role in the association of similarly charged particals. It was shown that besides this effect of chain fragments of 2-methyl-5-vinylpyridine a significant contribution in the association is made by non-polar interactions, created by methyl groups in copolymer 2-methyl-5-vinylpyridine and methacrylic acid. The association mechanism and structure of the formed complexes was studied. It was shown that complex formation is accompanied by non-equal distribution of the chain copolymer between the protein globules and appearance of asymmetric particles of the complex. A correlation between the ability of polyampholytes to bind protein and their physiological action was found.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008689	Methacrylates	Acrylic acids or acrylates which are substituted in the C-2 position with a methyl group.	Methacrylate
D008854	Microscopy, Electron	Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.	Electron Microscopy
D011108	Polymers	Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).	Polymer
D011145	Polyvinyls	POLYMERS derived from the monomer VINYL COMPOUNDS.	Polyvinyl
D011485	Protein Binding	The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.	Plasma Protein Binding Capacity,Binding, Protein
D000179	Acrylates	Derivatives of acrylic acid (the structural formula CH2
D012710	Serum Albumin, Bovine	Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)	Fetal Bovine Serum,Fetal Calf Serum,Albumin Bovine,Bovine Albumin,Bovine Serum Albumin,Albumin, Bovine,Albumin, Bovine Serum,Bovine Serum, Fetal,Bovine, Albumin,Calf Serum, Fetal,Serum, Fetal Bovine,Serum, Fetal Calf
D012995	Solubility	The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Solubilities
D013329	Structure-Activity Relationship	The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.	Relationship, Structure-Activity,Relationships, Structure-Activity,Structure Activity Relationship,Structure-Activity Relationships