Influence of the NaCl or CaCl2 concentration on the structure of heat-set bovine serum albumin gels at pH 7. 2005

Laurence Donato, and Catherine Garnier, and Jean-Louis Doublier, and Taco Nicolai
Unité de Physicochimie des Macromolécules, INRA, Rue de la Géraudière, BP 71642, 44316 Nantes Cedex 03, France.

The structure of heat-set systems of the globular protein bovine serum albumin (BSA) was investigated at pH 7 in different salt conditions (NaCl or CaCl(2)) using light scattering. Cross-correlation dynamic light scattering was used to correct for multiple scattering from turbid samples. After heat treatment, aggregates are formed whose size increases as the protein concentration increases. Beyond a critical concentration that decreases with increasing salt concentration, gels are formed. The heterogeneity and the reduced turbidity of the gels were found to increase with increasing salt concentration and to decrease with increasing protein concentration. The structure of the gels is determined by the strength of the repulsive electrostatic interactions between the aggregated proteins. The results obtained in NaCl are similar to those reported in previous studies for other globular proteins. CaCl(2) was found to be much more efficient in reducing electrostatic interactions than NaCl at the same ionic strength.

UI MeSH Term Description Entries
D008027 Light That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range. Light, Visible,Photoradiation,Radiation, Visible,Visible Radiation,Photoradiations,Radiations, Visible,Visible Light,Visible Radiations
D009391 Nephelometry and Turbidimetry Chemical analysis based on the phenomenon whereby light, passing through a medium with dispersed particles of a different refractive index from that of the medium, is attenuated in intensity by scattering. In turbidimetry, the intensity of light transmitted through the medium, the unscattered light, is measured. In nephelometry, the intensity of the scattered light is measured, usually, but not necessarily, at right angles to the incident light beam. Turbidimetry,Nephelometry,Turbidimetry and Nephelometry
D011487 Protein Conformation The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain). Conformation, Protein,Conformations, Protein,Protein Conformations
D002122 Calcium Chloride A salt used to replenish calcium levels, as an acid-producing diuretic, and as an antidote for magnesium poisoning. Calcium Chloride Dihydrate,Calcium Chloride, Anhydrous
D006358 Hot Temperature Presence of warmth or heat or a temperature notably higher than an accustomed norm. Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D012542 Scattering, Radiation The diversion of RADIATION (thermal, electromagnetic, or nuclear) from its original path as a result of interactions or collisions with atoms, molecules, or larger particles in the atmosphere or other media. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Radiation Scattering,Radiation Scatterings,Scatterings, Radiation
D012710 Serum Albumin, Bovine Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed) Fetal Bovine Serum,Fetal Calf Serum,Albumin Bovine,Bovine Albumin,Bovine Serum Albumin,Albumin, Bovine,Albumin, Bovine Serum,Bovine Serum, Fetal,Bovine, Albumin,Calf Serum, Fetal,Serum, Fetal Bovine,Serum, Fetal Calf
D012965 Sodium Chloride A ubiquitous sodium salt that is commonly used to season food. Sodium Chloride, (22)Na,Sodium Chloride, (24)NaCl
D055672 Static Electricity The accumulation of an electric charge on a object Electrostatic,Electrostatics,Static Charge,Charge, Static,Charges, Static,Electricity, Static,Static Charges

Related Publications

Laurence Donato, and Catherine Garnier, and Jean-Louis Doublier, and Taco Nicolai
February 1966, Biochemistry,
Laurence Donato, and Catherine Garnier, and Jean-Louis Doublier, and Taco Nicolai
December 2003, Journal of agricultural and food chemistry,
Laurence Donato, and Catherine Garnier, and Jean-Louis Doublier, and Taco Nicolai
February 2021, Food chemistry,
Laurence Donato, and Catherine Garnier, and Jean-Louis Doublier, and Taco Nicolai
January 2005, Journal of biomaterials science. Polymer edition,
Laurence Donato, and Catherine Garnier, and Jean-Louis Doublier, and Taco Nicolai
December 1973, Biochimica et biophysica acta,
Laurence Donato, and Catherine Garnier, and Jean-Louis Doublier, and Taco Nicolai
October 1961, Bollettino della Societa italiana di biologia sperimentale,
Laurence Donato, and Catherine Garnier, and Jean-Louis Doublier, and Taco Nicolai
January 1970, Biokhimiia (Moscow, Russia),
Laurence Donato, and Catherine Garnier, and Jean-Louis Doublier, and Taco Nicolai
August 1971, Biochimica et biophysica acta,
Laurence Donato, and Catherine Garnier, and Jean-Louis Doublier, and Taco Nicolai
May 1974, Analytical biochemistry,
Laurence Donato, and Catherine Garnier, and Jean-Louis Doublier, and Taco Nicolai
January 2003, International journal of biological macromolecules,
Copied contents to your clipboard!