BIOMAT Database Logo BIOMAT Database Logo

Expression profile and clonality of T-cell receptor beta variable genes in normal human endometrium. 2006

Pouneh Dokouhaki, and Rosa Moghadam, and Firoozeh Akbariasbagh, and Amirhassan Zarnani, and Marefat Ghaffari Novin, and Alireza Razavi, and Mahmood Jeddi-Tehrani
Department of Immunology, Reproductive Biotechnology Center, Avesina Research Institute, Tehran, Iran.

OBJECTIVE In spite of their key immunological role, alphabeta+ T cells residing in endometrium have not been extensively explored. We analyzed here expression profile of TCRBV genes in normal human endometrium compared with peripheral blood. METHODS Samples were taken from normal reproductive women. RT-PCR using BV-gene specific primers was performed on blood and endometrial samples. After blotting, hybridization with radiolabelled probe and autoradiography, relative expression of each TCRBV family was determined. Clonal expansions of the over-expressed genes were assessed by CDR3 length polymorphism. RESULTS Only one gene (TCRBV7) was expressed significantly and two other genes marginally more in the endometrium compared with blood. All three TCRBV genes examined showed a rather restricted pattern in the endometrium in contrast to polyclonal patterns in the blood. CONCLUSIONS Our results stress the similarities between T cells residing in different mucosal tissues and provide a basis for future investigations about endometrial T cells and their antigen specificities in gynecological problems.

UI MeSH Term Description Entries
D011110 Polymorphism, Genetic The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level. Gene Polymorphism,Genetic Polymorphism,Polymorphism (Genetics),Genetic Polymorphisms,Gene Polymorphisms,Polymorphism, Gene,Polymorphisms (Genetics),Polymorphisms, Gene,Polymorphisms, Genetic
D004717 Endometrium The mucous membrane lining of the uterine cavity that is hormonally responsive during the MENSTRUAL CYCLE and PREGNANCY. The endometrium undergoes cyclic changes that characterize MENSTRUATION. After successful FERTILIZATION, it serves to sustain the developing embryo. Endometria
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D013601 T-Lymphocytes Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen. T Cell,T Lymphocyte,T-Cells,Thymus-Dependent Lymphocytes,Cell, T,Cells, T,Lymphocyte, T,Lymphocyte, Thymus-Dependent,Lymphocytes, T,Lymphocytes, Thymus-Dependent,T Cells,T Lymphocytes,T-Cell,T-Lymphocyte,Thymus Dependent Lymphocytes,Thymus-Dependent Lymphocyte
D018076 DNA, Complementary Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe. Complementary DNA,cDNA,cDNA Probes,Probes, cDNA
D019674 Genes, T-Cell Receptor beta DNA sequences encoding the beta chain of the T-cell receptor. The genomic organization of the TcR beta genes is essentially the same in all species and is similar to the organization of Ig genes. T-Cell Receptor beta Genes,TcR beta Genes,T-Cell Receptor beta-Chain Genes,Gene, TcR beta,Genes, T Cell Receptor beta,Genes, TcR beta,T Cell Receptor beta Chain Genes,T Cell Receptor beta Genes,TcR beta Gene
D020869 Gene Expression Profiling The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell. Gene Expression Analysis,Gene Expression Pattern Analysis,Transcript Expression Analysis,Transcriptome Profiling,Transcriptomics,mRNA Differential Display,Gene Expression Monitoring,Transcriptome Analysis,Analyses, Gene Expression,Analyses, Transcript Expression,Analyses, Transcriptome,Analysis, Gene Expression,Analysis, Transcript Expression,Analysis, Transcriptome,Differential Display, mRNA,Differential Displays, mRNA,Expression Analyses, Gene,Expression Analysis, Gene,Gene Expression Analyses,Gene Expression Monitorings,Gene Expression Profilings,Monitoring, Gene Expression,Monitorings, Gene Expression,Profiling, Gene Expression,Profiling, Transcriptome,Profilings, Gene Expression,Profilings, Transcriptome,Transcript Expression Analyses,Transcriptome Analyses,Transcriptome Profilings,mRNA Differential Displays

Related Publications

Pouneh Dokouhaki, and Rosa Moghadam, and Firoozeh Akbariasbagh, and Amirhassan Zarnani, and Marefat Ghaffari Novin, and Alireza Razavi, and Mahmood Jeddi-Tehrani
Oligoclonal expression of T-cell receptor Beta variable genes in normal human endometrium.
June 2005, Iranian journal of allergy, asthma, and immunology,
Pouneh Dokouhaki, and Rosa Moghadam, and Firoozeh Akbariasbagh, and Amirhassan Zarnani, and Marefat Ghaffari Novin, and Alireza Razavi, and Mahmood Jeddi-Tehrani
Expression of T-cell receptor alpha and beta variable genes in normal and malignant human T cells.
May 1993, British journal of haematology,
Pouneh Dokouhaki, and Rosa Moghadam, and Firoozeh Akbariasbagh, and Amirhassan Zarnani, and Marefat Ghaffari Novin, and Alireza Razavi, and Mahmood Jeddi-Tehrani
Repertoire and clonality of T-cell receptor beta variable genes expressed in endometrium and blood T cells of patients with recurrent spontaneous abortion.
August 2008, American journal of reproductive immunology (New York, N.Y. : 1989),
Pouneh Dokouhaki, and Rosa Moghadam, and Firoozeh Akbariasbagh, and Amirhassan Zarnani, and Marefat Ghaffari Novin, and Alireza Razavi, and Mahmood Jeddi-Tehrani
The human T cell receptor beta variable (TRBV) genes.
January 2000, Experimental and clinical immunogenetics,
Pouneh Dokouhaki, and Rosa Moghadam, and Firoozeh Akbariasbagh, and Amirhassan Zarnani, and Marefat Ghaffari Novin, and Alireza Razavi, and Mahmood Jeddi-Tehrani
T-cell receptor variable beta genes show differential expression in CD4 and CD8 T cells.
November 1991, Human immunology,
Pouneh Dokouhaki, and Rosa Moghadam, and Firoozeh Akbariasbagh, and Amirhassan Zarnani, and Marefat Ghaffari Novin, and Alireza Razavi, and Mahmood Jeddi-Tehrani
Diversity and structure of human T-cell receptor beta-chain variable region genes.
September 1986, Proceedings of the National Academy of Sciences of the United States of America,
Pouneh Dokouhaki, and Rosa Moghadam, and Firoozeh Akbariasbagh, and Amirhassan Zarnani, and Marefat Ghaffari Novin, and Alireza Razavi, and Mahmood Jeddi-Tehrani
Sequences and diversity of human T cell receptor beta chain variable region genes.
September 1986, The Journal of experimental medicine,
Pouneh Dokouhaki, and Rosa Moghadam, and Firoozeh Akbariasbagh, and Amirhassan Zarnani, and Marefat Ghaffari Novin, and Alireza Razavi, and Mahmood Jeddi-Tehrani
T-cell receptor V beta expression in normal human skin.
February 1993, Proceedings of the National Academy of Sciences of the United States of America,
Pouneh Dokouhaki, and Rosa Moghadam, and Firoozeh Akbariasbagh, and Amirhassan Zarnani, and Marefat Ghaffari Novin, and Alireza Razavi, and Mahmood Jeddi-Tehrani
T-cell receptor beta-chain expression: dependence on relatively few variable region genes.
August 1985, Science (New York, N.Y.),
Pouneh Dokouhaki, and Rosa Moghadam, and Firoozeh Akbariasbagh, and Amirhassan Zarnani, and Marefat Ghaffari Novin, and Alireza Razavi, and Mahmood Jeddi-Tehrani
Sequences of four previously undescribed human T-cell receptor beta chain variable genes.
September 1991, Tissue antigens,
Copied contents to your clipboard!