Clostridium difficile and Clostridium perfringens species detected in infant faecal microbiota using 16S rRNA targeted probes. 2006

Matteo Fallani, and Lionel Rigottier-Gois, and Marga Aguilera, and Chantal Bridonneau, and Anne Collignon, and Christine A Edwards, and Gérard Corthier, and Joël Doré
Institut National de la Recherche Agronomique, Unité d'Ecologie et de Physiologie du Système Digestif, Jouy en Josas, France.

Clostridium perfringens and Clostridium difficile are pathogenic clostridia potentially associated with gastrointestinal infections and allergy in infants. To enable the molecular detection and quantification of these species in the infant gut, two 16S rRNA oligonucleotide probes were developed: Cdif198 for C. difficile and Cperf191 for C. perfringens. We defined the probes in silico using the RDP sequence database. The probes were then validated using FISH combined with flow cytometry and a collection of target and non-target strains, and faecal samples inoculated with dilutions of C. difficile and C. perfringens strains. These new probes were used to assess the composition of the intestinal microbiota of 33 infants of 1.5 to 18.5 months of age, associated with a panel of 8 probes targeting the predominant faecal bacterial groups of humans. The probes designed allowed detection and quantification of the relative proportions of C. difficile (0.5+/-1.0%) and C. perfringens (2.1+/-2.3%) in the microbiota of infants.

UI MeSH Term Description Entries
D007223 Infant A child between 1 and 23 months of age. Infants
D003015 Clostridium Infections Infections with bacteria of the genus CLOSTRIDIUM and closely related CLOSTRIDIOIDES species. Clostridioides Infections,Clostridioides difficile Infection,Clostridioides perfringens Food Poisoning,Clostridioides perfringens Infections,Clostridioides sordellii Infection,Clostridium difficile Infections,Clostridium sordellii Infections,Clostridium difficile Infection,Clostridium perfringens Food Poisoning,Clostridium perfringens Infections,Clostridium sordellii Infection,Infections, Clostridium,Clostridioides Infection,Clostridioides perfringens Infection,Clostridium Infection,Clostridium perfringens Infection,Infection, Clostridioides difficile,Infection, Clostridioides sordellii,Infection, Clostridium,Infection, Clostridium difficile,Infection, Clostridium sordellii
D003016 Clostridium perfringens The most common etiologic agent of GAS GANGRENE. It is differentiable into several distinct types based on the distribution of twelve different toxins. Clostridium welchii
D005243 Feces Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012336 RNA, Ribosomal, 16S Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis. 16S Ribosomal RNA,16S rRNA,RNA, 16S Ribosomal,Ribosomal RNA, 16S,rRNA, 16S
D015345 Oligonucleotide Probes Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin. Oligodeoxyribonucleotide Probes,Oligonucleotide Probe,Oligoribonucleotide Probes,Probe, Oligonucleotide,Probes, Oligodeoxyribonucleotide,Probes, Oligonucleotide,Probes, Oligoribonucleotide
D016360 Clostridioides difficile A common inhabitant of the colon flora in human infants and sometimes in adults. The type species Clostridioides difficile is formerly known as Clostridium difficile. It is a causative agent for CLOSTRIDIOIDES INFECTIONS and is associated with PSEUDOMEMBRANOUS ENTEROCOLITIS in patients receiving antibiotic therapy. Clostridium difficile
D017404 In Situ Hybridization, Fluorescence A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei. FISH Technique,Fluorescent in Situ Hybridization,Hybridization in Situ, Fluorescence,FISH Technic,Hybridization in Situ, Fluorescent,In Situ Hybridization, Fluorescent,FISH Technics,FISH Techniques,Technic, FISH,Technics, FISH,Technique, FISH,Techniques, FISH

Related Publications

Matteo Fallani, and Lionel Rigottier-Gois, and Marga Aguilera, and Chantal Bridonneau, and Anne Collignon, and Christine A Edwards, and Gérard Corthier, and Joël Doré
July 2005, Environmental microbiology,
Matteo Fallani, and Lionel Rigottier-Gois, and Marga Aguilera, and Chantal Bridonneau, and Anne Collignon, and Christine A Edwards, and Gérard Corthier, and Joël Doré
December 1988, Journal of clinical microbiology,
Matteo Fallani, and Lionel Rigottier-Gois, and Marga Aguilera, and Chantal Bridonneau, and Anne Collignon, and Christine A Edwards, and Gérard Corthier, and Joël Doré
February 2015, Infectious diseases (London, England),
Matteo Fallani, and Lionel Rigottier-Gois, and Marga Aguilera, and Chantal Bridonneau, and Anne Collignon, and Christine A Edwards, and Gérard Corthier, and Joël Doré
March 2014, International journal of clinical practice,
Matteo Fallani, and Lionel Rigottier-Gois, and Marga Aguilera, and Chantal Bridonneau, and Anne Collignon, and Christine A Edwards, and Gérard Corthier, and Joël Doré
April 2021, European journal of gastroenterology & hepatology,
Matteo Fallani, and Lionel Rigottier-Gois, and Marga Aguilera, and Chantal Bridonneau, and Anne Collignon, and Christine A Edwards, and Gérard Corthier, and Joël Doré
August 2014, Anaerobe,
Matteo Fallani, and Lionel Rigottier-Gois, and Marga Aguilera, and Chantal Bridonneau, and Anne Collignon, and Christine A Edwards, and Gérard Corthier, and Joël Doré
April 1992, International journal of systematic bacteriology,
Matteo Fallani, and Lionel Rigottier-Gois, and Marga Aguilera, and Chantal Bridonneau, and Anne Collignon, and Christine A Edwards, and Gérard Corthier, and Joël Doré
June 2016, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America,
Matteo Fallani, and Lionel Rigottier-Gois, and Marga Aguilera, and Chantal Bridonneau, and Anne Collignon, and Christine A Edwards, and Gérard Corthier, and Joël Doré
February 2014, Ugeskrift for laeger,
Matteo Fallani, and Lionel Rigottier-Gois, and Marga Aguilera, and Chantal Bridonneau, and Anne Collignon, and Christine A Edwards, and Gérard Corthier, and Joël Doré
May 2017, The Ulster medical journal,
Copied contents to your clipboard!