Cytotoxicity and cell signalling induced by continuous mild hyperthermia in freshly isolated mouse hepatocytes. 2006

Maria João Santos-Marques, and Félix Carvalho, and Carla Sousa, and Fernando Remião, and Rui Vitorino, and Francisco Amado, and Rita Ferreira, and José Alberto Duarte, and Maria de Lourdes Bastos
REQUIMTE, Toxicology Department, Faculty of Pharmacy, University of Porto, Rua Aníbal Cunha, 164, 4099-030 Porto, Portugal. mjsantosmarques@hotmail.com

An increasing body of data has been demonstrating that mammalian cells have elaborate networks of molecular signalling in counteracting heat shock and in developing adaptation to oxidative stress to avoid cell death. However, the precise mechanisms linking heat shock, oxidative stress and cell survival/cell death mechanisms are not yet clearly understood. The purpose of this study was thus to study the time course of hyperthermia-induced oxidative stress and cellular signalling through the activation of heat shock factor 1 (HSF1) and heat shock protein 70 (HSP70), using freshly isolated mouse hepatocytes. The results accomplished in this work demonstrated that mild continuous hyperthermia (41 degrees ) leads to oxidative stress and loss of cellular viability in a time-dependent manner, with significant effects already observed at the first hour of incubation. These toxic effects developed concomitantly with activation of HSF1 and emerged before the formation of HSP70 levels. Thus, although cell signalling was triggered through the transcriptional activation of HSP70 via HSF1, this putative protective process did not modify the trend of hepatotoxic effects mediated by this type of hyperthermic challenging.

UI MeSH Term Description Entries
D008297 Male Males
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004268 DNA-Binding Proteins Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. DNA Helix Destabilizing Proteins,DNA-Binding Protein,Single-Stranded DNA Binding Proteins,DNA Binding Protein,DNA Single-Stranded Binding Protein,SS DNA BP,Single-Stranded DNA-Binding Protein,Binding Protein, DNA,DNA Binding Proteins,DNA Single Stranded Binding Protein,DNA-Binding Protein, Single-Stranded,Protein, DNA-Binding,Single Stranded DNA Binding Protein,Single Stranded DNA Binding Proteins
D005978 Glutathione A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides. Reduced Glutathione,gamma-L-Glu-L-Cys-Gly,gamma-L-Glutamyl-L-Cysteinylglycine,Glutathione, Reduced,gamma L Glu L Cys Gly,gamma L Glutamyl L Cysteinylglycine
D006358 Hot Temperature Presence of warmth or heat or a temperature notably higher than an accustomed norm. Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D000076249 Heat Shock Transcription Factors Heat and cold stress-inducible, transcription factors that bind to inverted 5'-NGAAN-3' pentamer DNA sequences and are regulated by POLY-ADP-RIBOSYLATION. They play essential roles as transcriptional activators of the HEAT-SHOCK RESPONSE by inducing expression of large classes of MOLECULAR CHAPERONES and heat-shock proteins. They also function in DNA REPAIR; transcriptional reactivation of latent HIV-1; and pre-mRNA processing and nuclear export of HSP70 HEAT-SHOCK PROTEINS during heat stress. Heat Stress Transcription Factor,Plant Heat Shock Factor,Heat Shock Factor Protein 1,Heat Shock Factor, Plant,Heat Shock Transcription Factor,Heat Shock Transcription Factor 1,Heat Stress Transcription Factors,Plant Heat Shock Factors
D000222 Adaptation, Physiological The non-genetic biological changes of an organism in response to challenges in its ENVIRONMENT. Adaptation, Physiologic,Adaptations, Physiologic,Adaptations, Physiological,Adaptive Plasticity,Phenotypic Plasticity,Physiological Adaptation,Physiologic Adaptation,Physiologic Adaptations,Physiological Adaptations,Plasticity, Adaptive,Plasticity, Phenotypic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor

Related Publications

Maria João Santos-Marques, and Félix Carvalho, and Carla Sousa, and Fernando Remião, and Rui Vitorino, and Francisco Amado, and Rita Ferreira, and José Alberto Duarte, and Maria de Lourdes Bastos
January 2015, Research in pharmaceutical sciences,
Maria João Santos-Marques, and Félix Carvalho, and Carla Sousa, and Fernando Remião, and Rui Vitorino, and Francisco Amado, and Rita Ferreira, and José Alberto Duarte, and Maria de Lourdes Bastos
February 2001, Archives of toxicology,
Maria João Santos-Marques, and Félix Carvalho, and Carla Sousa, and Fernando Remião, and Rui Vitorino, and Francisco Amado, and Rita Ferreira, and José Alberto Duarte, and Maria de Lourdes Bastos
June 2013, Journal of biochemical and molecular toxicology,
Maria João Santos-Marques, and Félix Carvalho, and Carla Sousa, and Fernando Remião, and Rui Vitorino, and Francisco Amado, and Rita Ferreira, and José Alberto Duarte, and Maria de Lourdes Bastos
June 2006, Chemico-biological interactions,
Maria João Santos-Marques, and Félix Carvalho, and Carla Sousa, and Fernando Remião, and Rui Vitorino, and Francisco Amado, and Rita Ferreira, and José Alberto Duarte, and Maria de Lourdes Bastos
April 1998, Toxicology and applied pharmacology,
Maria João Santos-Marques, and Félix Carvalho, and Carla Sousa, and Fernando Remião, and Rui Vitorino, and Francisco Amado, and Rita Ferreira, and José Alberto Duarte, and Maria de Lourdes Bastos
January 1998, BioFactors (Oxford, England),
Maria João Santos-Marques, and Félix Carvalho, and Carla Sousa, and Fernando Remião, and Rui Vitorino, and Francisco Amado, and Rita Ferreira, and José Alberto Duarte, and Maria de Lourdes Bastos
November 1980, Biochemical pharmacology,
Maria João Santos-Marques, and Félix Carvalho, and Carla Sousa, and Fernando Remião, and Rui Vitorino, and Francisco Amado, and Rita Ferreira, and José Alberto Duarte, and Maria de Lourdes Bastos
July 1995, Chemico-biological interactions,
Maria João Santos-Marques, and Félix Carvalho, and Carla Sousa, and Fernando Remião, and Rui Vitorino, and Francisco Amado, and Rita Ferreira, and José Alberto Duarte, and Maria de Lourdes Bastos
November 2016, Drug research,
Maria João Santos-Marques, and Félix Carvalho, and Carla Sousa, and Fernando Remião, and Rui Vitorino, and Francisco Amado, and Rita Ferreira, and José Alberto Duarte, and Maria de Lourdes Bastos
November 2007, Journal of toxicology and environmental health. Part A,
Copied contents to your clipboard!