Biomaterial Database

Uptake and retrograde transport of [3H]GABA from the cochlear nucleus to the superior olive in the guinea pig. 1990

E M Ostapoff, and D K Morest, and S J Potashner

Department of Anatomy, University of Connecticut Health Center, Farmington 06032.

The purpose of the present study is to determine which descending projections to the cochlear nucleus may use gamma-aminobutyric acid (GABA) as a neurotransmitter. [3H]GABA (120 microM) was injected into the cochlear nucleus of albino and pigmented guinea pigs. After survival times between 0.25 and 16 h, the brain stems were prepared for light microscopic autoradiography. After 2 h survival there was a pulse of label, which progressed through the fibres from the cochlear nucleus to the ipsilateral superior olive. After 5 h, retrogradely labelled neuronal cell bodies and fibres were located in the superior olivary complex bilaterally. In the trapezoid body, clusters of labelled cells were seen in the lateral nucleus, ipsilaterally, and in the ventral nucleus, bilaterally. Also there were labelled cells in the ipsilateral dorsal and anterolateral periolivary nucleus. Large and small cells of several types were labelled. Survival times of 10 h or more resulted in very light, diffuse labelling. Projections to the cochlear nucleus labelled by retrograde transport of horseradish peroxidase that did not take up [3H]GABA included the inferior colliculus, bilaterally, and the cochlear nucleus and periolivary nuclei (other than ventral trapezoid nucleus), contralaterally. The selective labelling of cell groups in the superior olive with the moderately low concentration of [3H]GABA used is consistent with the high-affinity uptake of [3H]GABA by synaptic endings in the cochlear nucleus and its retrograde by transport GABA-ergic neurons. This provides evidence for a descending projection system for inhibitory feedback from the superior olive to the cochlear nucleus.

UI	MeSH Term	Description	Entries
D009434	Neural Pathways	Neural tracts connecting one part of the nervous system with another.	Neural Interconnections,Interconnection, Neural,Interconnections, Neural,Neural Interconnection,Neural Pathway,Pathway, Neural,Pathways, Neural
D009847	Olivary Nucleus	A brainstem nuclear complex. in the hindbrain, also referred to as the olivary body. The olivary nuclear complex is a part of the MEDULLA OBLONGATA and the PONTINE TEGMENTUM. It is involved with motor control and is a major source of sensory input to the CEREBELLUM.	Basal Nucleus, Olivary,Nucleus Basalis, Olivary,Olivary Body,Olivary Complex,Olivary Nuclei,Complex, Olivary,Nucleus, Olivary,Nucleus, Olivary Basal,Olivary Basal Nucleus,Olivary Bodies
D003056	Cochlear Nerve	The cochlear part of the 8th cranial nerve (VESTIBULOCOCHLEAR NERVE). The cochlear nerve fibers originate from neurons of the SPIRAL GANGLION and project peripherally to cochlear hair cells and centrally to the cochlear nuclei (COCHLEAR NUCLEUS) of the BRAIN STEM. They mediate the sense of hearing.	Acoustic Nerve,Auditory Nerve,Acoustic Nerves,Auditory Nerves,Cochlear Nerves,Nerve, Acoustic,Nerve, Auditory,Nerve, Cochlear,Nerves, Acoustic,Nerves, Auditory,Nerves, Cochlear
D005680	gamma-Aminobutyric Acid	The most common inhibitory neurotransmitter in the central nervous system.	4-Aminobutyric Acid,GABA,4-Aminobutanoic Acid,Aminalon,Aminalone,Gammalon,Lithium GABA,gamma-Aminobutyric Acid, Calcium Salt (2:1),gamma-Aminobutyric Acid, Hydrochloride,gamma-Aminobutyric Acid, Monolithium Salt,gamma-Aminobutyric Acid, Monosodium Salt,gamma-Aminobutyric Acid, Zinc Salt (2:1),4 Aminobutanoic Acid,4 Aminobutyric Acid,Acid, Hydrochloride gamma-Aminobutyric,GABA, Lithium,Hydrochloride gamma-Aminobutyric Acid,gamma Aminobutyric Acid,gamma Aminobutyric Acid, Hydrochloride,gamma Aminobutyric Acid, Monolithium Salt,gamma Aminobutyric Acid, Monosodium Salt
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D006651	Histocytochemistry	Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.	Cytochemistry
D006735	Horseradish Peroxidase	An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.	Alpha-Peroxidase,Ferrihorseradish Peroxidase,Horseradish Peroxidase II,Horseradish Peroxidase III,Alpha Peroxidase,II, Horseradish Peroxidase,III, Horseradish Peroxidase,Peroxidase II, Horseradish,Peroxidase III, Horseradish,Peroxidase, Ferrihorseradish,Peroxidase, Horseradish
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001345	Autoradiography	The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)	Radioautography
D001370	Axonal Transport	The directed transport of ORGANELLES and molecules along nerve cell AXONS. Transport can be anterograde (from the cell body) or retrograde (toward the cell body). (Alberts et al., Molecular Biology of the Cell, 3d ed, pG3)	Axoplasmic Flow,Axoplasmic Transport,Axoplasmic Streaming,Axonal Transports,Axoplasmic Flows,Axoplasmic Transports,Streaming, Axoplasmic,Transport, Axonal,Transport, Axoplasmic,Transports, Axonal,Transports, Axoplasmic