Cell cycle-dependent expression of gamma-tubulin in the amicronuclear ciliate Tetrahymena pyriformis. 2007

Ewa Joachimiak, and Sandra Pucciarelli, and Sabrina Barchetta, and Patrizia Ballarini, and Janina Kaczanowska, and Cristina Miceli
Department of Molecular, Cellular and Animal Biology, University of Camerino, Camerino 62032, v. Camerini 2, Italy.

In ciliates, different microtubular structures are nucleated from diverse Microtubule Organizing Centers (MTOCs). gamma-Tubulin is a tubulin superfamily member that plays an essential role in microtubule nucleation at the MTOCs. However, little is known about mechanisms regulating the activity of gamma-tubulin on different MTOCs and during the cell cycle. In Tetrahymena thermophila, the alpha- and beta-tubulin expression is regulated mainly at the transcriptional level, and changes in the ratio of polymerized/unpolymerized tubulin dimers lead to an increase or decrease of alpha- and beta-tubulin transcription. This study deals with the characterization of gamma-tubulin in the amicronuclear ciliate Tetrahymena pyriformis. Sequence analysis revealed some specific substitutions in nucleotide-binding loops characteristic of the Tetrahymena genus and putative conserved phosphorylation sites located on the external surface of the gamma-tubulin molecule. gamma-Tubulin expression during the cell cycle, in the presence of microtubular poisons and after deciliation, was also characterized. We found that gamma-tubulin mRNA levels are correlated with basal body proliferation and gamma-tubulin nuclear localization. We also found that gamma-tubulin expression changes during anti-microtubular drugs treatment, but does not changes during reciliation. These findings suggest a relationship between the level of unpolymerized tubulin dimers and gamma-tubulin transcription.

UI MeSH Term Description Entries
D008958 Models, Molecular Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures. Molecular Models,Model, Molecular,Molecular Model
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002453 Cell Cycle The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE. Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013769 Tetrahymena pyriformis A species of ciliate protozoa used extensively in genetic research. Tetrahymena pyriformi,pyriformi, Tetrahymena
D014404 Tubulin A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE. alpha-Tubulin,beta-Tubulin,delta-Tubulin,epsilon-Tubulin,gamma-Tubulin,alpha Tubulin,beta Tubulin,delta Tubulin,epsilon Tubulin,gamma Tubulin
D015800 Protozoan Proteins Proteins found in any species of protozoan. Proteins, Protozoan
D016054 DNA, Protozoan Deoxyribonucleic acid that makes up the genetic material of protozoa. Protozoan DNA
D017422 Sequence Analysis, DNA A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis. DNA Sequence Analysis,Sequence Determination, DNA,Analysis, DNA Sequence,DNA Sequence Determination,DNA Sequence Determinations,DNA Sequencing,Determination, DNA Sequence,Determinations, DNA Sequence,Sequence Determinations, DNA,Analyses, DNA Sequence,DNA Sequence Analyses,Sequence Analyses, DNA,Sequencing, DNA

Related Publications

Ewa Joachimiak, and Sandra Pucciarelli, and Sabrina Barchetta, and Patrizia Ballarini, and Janina Kaczanowska, and Cristina Miceli
November 2013, European journal of protistology,
Ewa Joachimiak, and Sandra Pucciarelli, and Sabrina Barchetta, and Patrizia Ballarini, and Janina Kaczanowska, and Cristina Miceli
December 1983, FEBS letters,
Ewa Joachimiak, and Sandra Pucciarelli, and Sabrina Barchetta, and Patrizia Ballarini, and Janina Kaczanowska, and Cristina Miceli
July 1969, Transactions of the American Microscopical Society,
Ewa Joachimiak, and Sandra Pucciarelli, and Sabrina Barchetta, and Patrizia Ballarini, and Janina Kaczanowska, and Cristina Miceli
June 1967, Proceedings of the National Academy of Sciences of the United States of America,
Ewa Joachimiak, and Sandra Pucciarelli, and Sabrina Barchetta, and Patrizia Ballarini, and Janina Kaczanowska, and Cristina Miceli
February 1971, Biochimica et biophysica acta,
Ewa Joachimiak, and Sandra Pucciarelli, and Sabrina Barchetta, and Patrizia Ballarini, and Janina Kaczanowska, and Cristina Miceli
July 1983, FEBS letters,
Ewa Joachimiak, and Sandra Pucciarelli, and Sabrina Barchetta, and Patrizia Ballarini, and Janina Kaczanowska, and Cristina Miceli
January 1991, DNA sequence : the journal of DNA sequencing and mapping,
Ewa Joachimiak, and Sandra Pucciarelli, and Sabrina Barchetta, and Patrizia Ballarini, and Janina Kaczanowska, and Cristina Miceli
December 1965, Nature,
Ewa Joachimiak, and Sandra Pucciarelli, and Sabrina Barchetta, and Patrizia Ballarini, and Janina Kaczanowska, and Cristina Miceli
February 1984, Cell calcium,
Ewa Joachimiak, and Sandra Pucciarelli, and Sabrina Barchetta, and Patrizia Ballarini, and Janina Kaczanowska, and Cristina Miceli
January 1972, Tsitologiia,
Copied contents to your clipboard!