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The RepA protein of plasmid R1 is rate-limiting for initiation of R1 replication. Its synthesis is mainly regulated by interactions of the antisense RNA, CopA, with the leader region of the RepA mRNA, CopT. This work describes the characterization of several mutants with sequence alterations in the intergenic region between the copA gene and the repA reading frame. The analysis showed that most of the mutations led both to a decrease in stability of maintenance of mini-R1 derivatives and to lowered repA expression assayed in translational repA-lacZ fusion constructs. Destruction of the copA gene and replacement of the upstream region by the tac promoter in the latter constructs indicated that these mutations per se alter the expression of repA. In addition, we show that particular mutations in this region can directly affect CopA-mediated control, either by changing the kinetics of interaction of CopA RNA with the RepA mRNA and/or by modifying the activity of the copA promoter. These data indicate the importance of the region analysed in the process that controls R1 replication.
A Berzal-Herranz, and
E G Wagner, and
R Díaz-Orejas
November 1991,
Molecular & general genetics : MGG,
A Berzal-Herranz, and
E G Wagner, and
R Díaz-Orejas
July 1992,
The EMBO journal,
A Berzal-Herranz, and
E G Wagner, and
R Díaz-Orejas
February 1987,
The EMBO journal,
A Berzal-Herranz, and
E G Wagner, and
R Díaz-Orejas
March 1992,
The EMBO journal,
A Berzal-Herranz, and
E G Wagner, and
R Díaz-Orejas
October 1988,
The EMBO journal,
A Berzal-Herranz, and
E G Wagner, and
R Díaz-Orejas
April 1989,
Nucleic acids research,
A Berzal-Herranz, and
E G Wagner, and
R Díaz-Orejas
July 1998,
Microbiology (Reading, England),
A Berzal-Herranz, and
E G Wagner, and
R Díaz-Orejas
January 2006,
Plasmid,
A Berzal-Herranz, and
E G Wagner, and
R Díaz-Orejas
January 1982,
The EMBO journal,
A Berzal-Herranz, and
E G Wagner, and
R Díaz-Orejas
April 1994,
Molecular microbiology,
