-transparent.png){kind=logo}
Antigenic properties of a murine transgenic model for hereditary retinoblastoma, induced by a chimeric gene coding for Simian virus 40 large T antigen, an oncogene that inactivates the retinoblastoma susceptibility gene product, were studied by immunohistochemistry. All transgenic mice develop bilateral intraocular retinal tumors in the inner nuclear layer with Homer Wright-like rosettes, and one quarter develop midbrain tumors resembling trilateral retinoblastoma. Cell lines TE-1 and TM-1 were established from intraocular and metastatic tumors, respectively. Intraocular tumors reacted with antibodies to neuron-specific enolase and synaptophysin, while vimentin, glial fibrillary acidic, and S-100 proteins were detected only in reactive glia derived from adjacent retina. The midbrain tumors showed weak reactivity to synaptophysin, and they blended with reactive astrocytes positive for glial markers. The tumors were negative for cytokeratins. Finally both derived cell lines expressed synaptophysin and individual neurofilament triplet proteins in immunofluorescence and Western blotting, supporting their essentially neuronal nature. The antigenic profile resembles human retinoblastoma, but differences in morphology and antigen distribution suggest a more close relationship to neurons of the inner nuclear layer than to photoreceptor cells.
T Kivelä, and
I Virtanen, and
D M Marcus, and
J M O'Brien, and
J L Carpenter, and
E Brauner, and
A Tarkkanen, and
D M Albert
September 2005,
The British journal of ophthalmology,
T Kivelä, and
I Virtanen, and
D M Marcus, and
J M O'Brien, and
J L Carpenter, and
E Brauner, and
A Tarkkanen, and
D M Albert
August 1984,
Experimental eye research,
T Kivelä, and
I Virtanen, and
D M Marcus, and
J M O'Brien, and
J L Carpenter, and
E Brauner, and
A Tarkkanen, and
D M Albert
January 1984,
Virchows Archiv. A, Pathological anatomy and histopathology,
T Kivelä, and
I Virtanen, and
D M Marcus, and
J M O'Brien, and
J L Carpenter, and
E Brauner, and
A Tarkkanen, and
D M Albert
August 1995,
The British journal of ophthalmology,
T Kivelä, and
I Virtanen, and
D M Marcus, and
J M O'Brien, and
J L Carpenter, and
E Brauner, and
A Tarkkanen, and
D M Albert
August 1996,
Investigative ophthalmology & visual science,
T Kivelä, and
I Virtanen, and
D M Marcus, and
J M O'Brien, and
J L Carpenter, and
E Brauner, and
A Tarkkanen, and
D M Albert
August 1990,
Archives of ophthalmology (Chicago, Ill. : 1960),
T Kivelä, and
I Virtanen, and
D M Marcus, and
J M O'Brien, and
J L Carpenter, and
E Brauner, and
A Tarkkanen, and
D M Albert
January 1995,
Investigative ophthalmology & visual science,
T Kivelä, and
I Virtanen, and
D M Marcus, and
J M O'Brien, and
J L Carpenter, and
E Brauner, and
A Tarkkanen, and
D M Albert
January 2020,
Frontiers in neuroscience,
T Kivelä, and
I Virtanen, and
D M Marcus, and
J M O'Brien, and
J L Carpenter, and
E Brauner, and
A Tarkkanen, and
D M Albert
January 2016,
Arquivos brasileiros de oftalmologia,
T Kivelä, and
I Virtanen, and
D M Marcus, and
J M O'Brien, and
J L Carpenter, and
E Brauner, and
A Tarkkanen, and
D M Albert
February 2004,
Archives of ophthalmology (Chicago, Ill. : 1960),
