-transparent.png){kind=logo}
Selenomethionine metabolism and the biochemical basis for its cytotoxicity were analyzed in cultured human and murine lymphoid cells. The metabolic pathways were also addressed, using purified mammalian enzymes and crude tissue extracts. Selenomethionine was found to be effectively metabolized to S-adenosylmethionine analog, and that analog was further metabolized in transmethylation reactions and in polyamine synthesis, similarly to the corresponding sulphur metabolites of methionine. Selenomethionine did not block these pathways, nor was there a specific block on the synthesis of DNA, RNA, or proteins when added to the culture medium. Selenomethionine showed cytotoxicity at above 40 microM levels. Yet, low selenomethionine levels (10 microM) could replace methionine and support cell growth in the absence of methionine. Selenomethionine toxicity took place concomitantly with changes in S-adenosylmethionine pools. D-form was less cytotoxic than L-form. Methionine concentration modified the cytotoxicity. Together, this indicates that selenomethionine uptake and enzymic metabolism are involved in the cytotoxicity in a yet unknown way.
E O Kajander, and
R J Harvima, and
T O Eloranta, and
H Martikainen, and
M Kantola, and
S O Kärenlampi, and
K Akerman
March 2007,
The Kaohsiung journal of medical sciences,
E O Kajander, and
R J Harvima, and
T O Eloranta, and
H Martikainen, and
M Kantola, and
S O Kärenlampi, and
K Akerman
August 1995,
Seminars in oncology,
E O Kajander, and
R J Harvima, and
T O Eloranta, and
H Martikainen, and
M Kantola, and
S O Kärenlampi, and
K Akerman
January 1980,
Journal of environmental pathology and toxicology,
E O Kajander, and
R J Harvima, and
T O Eloranta, and
H Martikainen, and
M Kantola, and
S O Kärenlampi, and
K Akerman
September 2000,
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine,
E O Kajander, and
R J Harvima, and
T O Eloranta, and
H Martikainen, and
M Kantola, and
S O Kärenlampi, and
K Akerman
October 1991,
Biochimica et biophysica acta,
E O Kajander, and
R J Harvima, and
T O Eloranta, and
H Martikainen, and
M Kantola, and
S O Kärenlampi, and
K Akerman
June 1986,
Journal of the National Cancer Institute,
E O Kajander, and
R J Harvima, and
T O Eloranta, and
H Martikainen, and
M Kantola, and
S O Kärenlampi, and
K Akerman
May 1986,
Toxicology and applied pharmacology,
E O Kajander, and
R J Harvima, and
T O Eloranta, and
H Martikainen, and
M Kantola, and
S O Kärenlampi, and
K Akerman
June 2017,
Toxicology in vitro : an international journal published in association with BIBRA,
E O Kajander, and
R J Harvima, and
T O Eloranta, and
H Martikainen, and
M Kantola, and
S O Kärenlampi, and
K Akerman
April 1989,
Toxicology,
E O Kajander, and
R J Harvima, and
T O Eloranta, and
H Martikainen, and
M Kantola, and
S O Kärenlampi, and
K Akerman
November 1968,
The British journal of surgery,
