Biomaterial Database

Cellular metabolism of lead: a kinetic analysis in cultured osteoclastic bone cells. 1986

J G Pounds, and J F Rosen

Detailed characterization of the modulation of lead metabolism in bone is necessary to understand the role of skeletal lead in the expression of clinical and biochemical effects of lead intoxication. The metabolism of lead in osseous tissue is also clinically important because it is the major site of chelation by therapeutic agents, such as CaNa2-EDTA and D-penicillamine. Experiments were conducted to characterize the steady-state kinetic distribution and behavior of 210Pb in osteoclastic bone cells and to identify the biological structures or functions associated with the kinetic pools. Bone cells, derived from mouse calvaria, were enriched for osteoclasts by a sequential collagenase digestion and maintained in primary culture for 1 week. Cultures were labeled with 210Pb as 5 microM lead acetate for 20 hr and the kinetic parameters were obtained by analysis of 210Pb washout curves. Cellular metabolism was defined by three kinetic pools of intracellular lead containing approximately 10% (S1). Approximately 12% (S2), and approximately 78% (S3) of total cellular lead (1.2 nmol/mg cell protein). The halftimes for isotopic exchange were 1, 27, and 480 min, respectively. These data indicate that lead is readily exchangeable from osteoclastic bone cells and, as in soft tissues (hepatocytes), the bulk of cellular lead is associated with mitochondria.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D007811	Lanthanum	The prototypical element in the rare earth family of metals. It has the atomic symbol La, atomic number 57, and atomic weight 138.91. Lanthanide ion is used in experimental biology as a calcium antagonist; lanthanum oxide improves the optical properties of glass.
D007854	Lead	A soft, grayish metal with poisonous salts; atomic number 82, atomic weight 207.2, symbol Pb.
D008858	Microscopy, Phase-Contrast	A form of interference microscopy in which variations of the refracting index in the object are converted into variations of intensity in the image. This is achieved by the action of a phase plate.	Phase-Contrast Microscopy,Microscopies, Phase-Contrast,Microscopy, Phase Contrast,Phase Contrast Microscopy,Phase-Contrast Microscopies
D008954	Models, Biological	Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.	Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D010010	Osteoclasts	A large multinuclear cell associated with the BONE RESORPTION. An odontoclast, also called cementoclast, is cytomorphologically the same as an osteoclast and is involved in CEMENTUM resorption.	Odontoclasts,Cementoclast,Cementoclasts,Odontoclast,Osteoclast
D010710	Phosphates	Inorganic salts of phosphoric acid.	Inorganic Phosphate,Phosphates, Inorganic,Inorganic Phosphates,Orthophosphate,Phosphate,Phosphate, Inorganic
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D004533	Egtazic Acid	A chelating agent relatively more specific for calcium and less toxic than EDETIC ACID.	EGTA,Ethylene Glycol Tetraacetic Acid,EGATA,Egtazic Acid Disodium Salt,Egtazic Acid Potassium Salt,Egtazic Acid Sodium Salt,Ethylene Glycol Bis(2-aminoethyl ether)tetraacetic Acid,Ethylenebis(oxyethylenenitrile)tetraacetic Acid,GEDTA,Glycoletherdiamine-N,N,N',N'-tetraacetic Acid,Magnesium-EGTA,Tetrasodium EGTA,Acid, Egtazic,EGTA, Tetrasodium,Magnesium EGTA
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia