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A6 cells, a continuous cell line derived from kidney of Xenopus laevis, were incubated with [3H]-dexamethasone for 24 h. When radioactive compounds in media were separated by reversed phase high pressure liquid chromatography, two radioactive fractions were found. The less polar fraction which contained 91-93% of total radioactivity cochromatographed with dexamethasone, whereas the polar fraction contained 5% of total radioactivity in media. In order to rigorously identify the polar metabolite, large scale cultures were carried out and the polar compound was separated and purified by reversed phase high pressure liquid chromatography. The purified material was analyzed by secondary ion mass spectrometry and nuclear magnetic resonance spectroscopy. By these procedures, this material was identified as 6 beta-hydroxydexamethasone. To our knowledge these are the first data indicating that dexamethasone can be metabolized by transporting epithelia such as A6 cells.
K Matsuzaki, and
T Miyazaki, and
K Yasuda
January 1986,
Steroids,
K Matsuzaki, and
T Miyazaki, and
K Yasuda
November 1990,
Steroids,
K Matsuzaki, and
T Miyazaki, and
K Yasuda
November 1992,
Biological psychiatry,
K Matsuzaki, and
T Miyazaki, and
K Yasuda
May 2004,
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
K Matsuzaki, and
T Miyazaki, and
K Yasuda
August 1995,
Pflugers Archiv : European journal of physiology,
K Matsuzaki, and
T Miyazaki, and
K Yasuda
June 1995,
Annals of the New York Academy of Sciences,
K Matsuzaki, and
T Miyazaki, and
K Yasuda
May 2009,
Biochemical and biophysical research communications,
K Matsuzaki, and
T Miyazaki, and
K Yasuda
July 1995,
Research in veterinary science,
K Matsuzaki, and
T Miyazaki, and
K Yasuda
July 2021,
Bioanalysis,
K Matsuzaki, and
T Miyazaki, and
K Yasuda
March 1990,
The American journal of physiology,
