Biomaterial Database

[SNPs of myostatin gene and its genetic effects on carcass traits in chicken]. 2007

Zhi Zhu, and Deng-Jun Wu, and Ning-Ying Xu

College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China.

Two polymorphic sites in the first exon 1 of MSTN gene in 180 Wenling grass-chicken including 3 lines were studied by PCR-RFLP method,and its genetic effects on carcass traits were researched. The results showed that 2 genotypes (MN and NN) in G(2100)A locus were identified by Bsh1236 I and 3 genotypes (AA, AB and BB) in G(2109)A locus were identified by Msp I, and 5 genotypes were emerged while com-analyzing 2 loci. The chi2 test indicated genotype frequencies had no significant deviation (P > 0.05) among lines. The analysis of variance showed that the different genotypes had significant deviation (P <0.01 or P < 0.05) in carcass percent. The multiple comparisons were made. The abdominal fat weight and carcass percent of heterozygosis MN were significantly higher (P<0.05) than that of mutant NN,and the breast muscle weight and breast muscle percent of heterozygosis AB were significantly higher (P < 0.01 or P<0.05) than that of AA individuals. In addition,the abdominal fat weight and abdominal fat percent of AA individuals were significantly higher (P<0.01) than that of mutant BB individuals,but BB individuals were significantly lower (P<0.05) than AA and AB individuals in leg muscle weight trait. The abdominal fat weight,abdominal fat percent and breast muscle percent of genotype NA/MA individuals were significantly higher or lower (P<0.01) than those in other genotype individuals while com-analyzing 2 loci.

UI	MeSH Term	Description	Entries
D009929	Organ Size	The measurement of an organ in volume, mass, or heaviness.	Organ Volume,Organ Weight,Size, Organ,Weight, Organ
D010641	Phenotype	The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.	Phenotypes
D012150	Polymorphism, Restriction Fragment Length	Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.	RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D001823	Body Composition	The relative amounts of various components in the body, such as percentage of body fat.	Body Compositions,Composition, Body,Compositions, Body
D002645	Chickens	Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.	Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D005787	Gene Frequency	The proportion of one particular in the total of all ALLELES for one genetic locus in a breeding POPULATION.	Allele Frequency,Genetic Equilibrium,Equilibrium, Genetic,Allele Frequencies,Frequencies, Allele,Frequencies, Gene,Frequency, Allele,Frequency, Gene,Gene Frequencies
D005838	Genotype	The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.	Genogroup,Genogroups,Genotypes
D000273	Adipose Tissue	Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white.	Fatty Tissue,Body Fat,Fat Pad,Fat Pads,Pad, Fat,Pads, Fat,Tissue, Adipose,Tissue, Fatty
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D016133	Polymerase Chain Reaction	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.	Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain