Biomaterial Database

[Chemopreventive effect of oxymatrine on N-methyl-N'-nitro-N-nitrosoguanidine induced gastrointestinal cancer: experiment with rats]. 2007

Qi Xu, and Xi-feng Jin, and Zhi-hua Ran, and Chuan-hua Yang, and Shu-dong Xiao

Department of Oncology, Renji Hospital, Jiaotong University, Shanghai 200001, China.

OBJECTIVE To investigate the chemopreventive effect of oxymatrine on the N-methyl-N-nitro-N-nitrosoguanidine (MNNG)-induced gastrointestinal cancer. METHODS Ninety-nine male Wistar rats were randomly allocated into four groups: MNNG treated group (36 rats, fed with MNNG for 28 weeks so as to establish rat model of gastrointestinal cancer), oxymatrine intervention group (n = 23, fed with MNNG and oxymatrine), negative control group (20 rats, fed with mixed feed), and oxymatrine control group (20 rats. fed with normal food and oxymatrine). 28 weeks later all the rats began to be fed with normal food for 4 weeks. two rats in the MNNG treated group were killed at the 28 th, 32 nd, and 40 th weeks of the experiment respectively to observe the tumor growth in the gastrointestinal tract. At the 44 th week all the rats were sacrificed and assessed for the presence of gastrointestinal tumor. Immunohistochemistry was used to detect the expression of Ki67, an antigen associated to the proliferation of nucleus. The cell apoptosis rate in the tumor tissue was detected by TUNEL method. RESULTS Experiment was completed in 92 rats (92.93%). The incidence of gastrointestinal tumor in the MNNG treated group was 58.62% (17/29), significantly higher than that in the oxymatrine treated group (30.43%, 7/23, P < 0.05). Duodenum tumor was found in 1 rat of the negative control group and no tumor was found in the oxymatrine control group. The average volume of tumor in the MNNG treated group was 2.56 (full range 49.5) cm(3), significantly larger than those of the oxymatrine treated group [0.03 (full range 0.009) cm(3)], and negative control group (0.5 cm(3)) (both P < 0.05). The incidence rates of gastrointestinal mucosal ulceration and dysplasia of the MNNG treated group were higher than those of the oxymatrine treated group (both P < 0.05). The expression rate of Ki67 in the tumor tissue of the MNNG treated group was (48 +/- 18)%, significantly higher than that of the oxymatrine treated group [(25 +/- 24)%, P < 0.05]. The apoptotic rate of tumor cell in the MNNG treated group was (11 +/- 7)%, significantly lower than that in the oxymatrine treated group [(30 +/- 16)%, P = 0.002]. CONCLUSIONS Inhibiting the development and inducing the apoptosis of gastrointestinal tumor induced by MNNG, oxymatrine can be used as a chemopreventive agent against gastrointestinal tumor.

UI	MeSH Term	Description	Entries
D007150	Immunohistochemistry	Histochemical localization of immunoreactive substances using labeled antibodies as reagents.	Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D008297	Male		Males
D008769	Methylnitronitrosoguanidine	A nitrosoguanidine derivative with potent mutagenic and carcinogenic properties.	Methylnitrosonitroguanidine,Nitrosomethylnitroguanidine,Nitrosonitromethylguanidine,MNNG,N-Methyl-N'-nitro-N-nitrosoguanidine,N Methyl N' nitro N nitrosoguanidine
D011807	Quinolizines
D011897	Random Allocation	A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects.	Randomization,Allocation, Random
D005753	Gastric Mucosa	Lining of the STOMACH, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. The surface cells produce MUCUS that protects the stomach from attack by digestive acid and enzymes. When the epithelium invaginates into the LAMINA PROPRIA at various region of the stomach (CARDIA; GASTRIC FUNDUS; and PYLORUS), different tubular gastric glands are formed. These glands consist of cells that secrete mucus, enzymes, HYDROCHLORIC ACID, or hormones.	Cardiac Glands,Gastric Glands,Pyloric Glands,Cardiac Gland,Gastric Gland,Gastric Mucosas,Gland, Cardiac,Gland, Gastric,Gland, Pyloric,Glands, Cardiac,Glands, Gastric,Glands, Pyloric,Mucosa, Gastric,Mucosas, Gastric,Pyloric Gland
D005770	Gastrointestinal Neoplasms	Tumors or cancer of the GASTROINTESTINAL TRACT, from the MOUTH to the ANAL CANAL.	Gastrointestinal Cancer,Cancer of Gastrointestinal Tract,Cancer of the Gastrointestinal Tract,Neoplasms, Gastrointestinal,Cancer, Gastrointestinal,Cancers, Gastrointestinal,Gastrointestinal Cancers,Gastrointestinal Neoplasm,Gastrointestinal Tract Cancer,Gastrointestinal Tract Cancers,Neoplasm, Gastrointestinal
D000470	Alkaloids	Organic nitrogenous bases. Many alkaloids of medical importance occur in the animal and vegetable kingdoms, and some have been synthesized. (Grant & Hackh's Chemical Dictionary, 5th ed)	Alkaloid,Plant Alkaloid,Plant Alkaloids,Alkaloid, Plant,Alkaloids, Plant
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D017208	Rats, Wistar	A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.	Wistar Rat,Rat, Wistar,Wistar Rats