BIOMAT Database Logo BIOMAT Database Logo

Vaccinia DNA ligase complements Saccharomyces cerevisiae cdc9, localizes in cytoplasmic factories and affects virulence and virus sensitivity to DNA damaging agents. 1991

S M Kerr, and L H Johnston, and M Odell, and S A Duncan, and K M Law, and G L Smith
Sir William Dunn School of Pathology, University of Oxford, UK.

The functional compatibility of vaccinia virus DNA ligase with eukaryotic counterparts was demonstrated by its ability to complement Saccharomyces cerevisiae cdc9. The vaccinia DNA ligase is a 63 kDa protein expressed early during infection that is non-essential for virus DNA replication and recombination in cultured cells. This implies complementation by a mammalian DNA ligase, yet no obvious recruitment of host DNA ligase I from the nucleus to the cytoplasm was observed during infection. An antiserum raised against a peptide conserved in eukaryotic DNA ligases identified the virus enzyme in discrete cytoplasmic 'factories', the sites of virus DNA synthesis, demonstrating immunological cross-reactivity between host DNA ligase I and the vaccinia enzyme. DNA ligase was not detected in the factories of a mutant virus lacking the ligase gene. Despite this, no difference in growth between wild-type (WT) and mutant virus was detectable even in Bloom's syndrome cells which have reduced DNA ligase I activity. However, DNA ligase negative virus showed an increased sensitivity to UV or bleomycin in cultured cells, and the importance of DNA ligase for virus virulence in vivo was demonstrated by the attenuated phenotype of the deletion mutant in intranasally infected mice.

UI MeSH Term Description Entries
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009153 Mutagens Chemical agents that increase the rate of genetic mutation by interfering with the function of nucleic acids. A clastogen is a specific mutagen that causes breaks in chromosomes. Clastogen,Clastogens,Genotoxin,Genotoxins,Mutagen
D011088 DNA Ligases Poly(deoxyribonucleotide):poly(deoxyribonucleotide)ligases. Enzymes that catalyze the joining of preformed deoxyribonucleotides in phosphodiester linkage during genetic processes during repair of a single-stranded break in duplex DNA. The class includes both EC 6.5.1.1 (ATP) and EC 6.5.1.2 (NAD). DNA Joinases,DNA Ligase,Polydeoxyribonucleotide Ligases,Polydeoxyribonucleotide Synthetases,T4 DNA Ligase,DNA Ligase, T4,Joinases, DNA,Ligase, DNA,Ligase, T4 DNA,Ligases, DNA,Ligases, Polydeoxyribonucleotide,Synthetases, Polydeoxyribonucleotide
D011995 Recombination, Genetic Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses. Genetic Recombination,Recombination,Genetic Recombinations,Recombinations,Recombinations, Genetic
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003593 Cytoplasm The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990) Protoplasm,Cytoplasms,Protoplasms
D004249 DNA Damage Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS. DNA Injury,DNA Lesion,DNA Lesions,Genotoxic Stress,Stress, Genotoxic,Injury, DNA,DNA Injuries
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D004285 Dogs The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065) Canis familiaris,Dog

Related Publications

S M Kerr, and L H Johnston, and M Odell, and S A Duncan, and K M Law, and G L Smith
Saccharomyces cerevisiae cdc9, a structural gene for yeast DNA ligase which complements Schizosaccharomyces pombe cdc17.
August 1983, European journal of biochemistry,
S M Kerr, and L H Johnston, and M Odell, and S A Duncan, and K M Law, and G L Smith
Saccharomyces cerevisiae cell cycle mutant cdc9 is defective in DNA ligase.
August 1978, Nature,
S M Kerr, and L H Johnston, and M Odell, and S A Duncan, and K M Law, and G L Smith
Regulation of CDC9, the Saccharomyces cerevisiae gene that encodes DNA ligase.
January 1985, Molecular and cellular biology,
S M Kerr, and L H Johnston, and M Odell, and S A Duncan, and K M Law, and G L Smith
Cellular DNA ligase I is recruited to cytoplasmic vaccinia virus factories and masks the role of the vaccinia ligase in viral DNA replication.
December 2009, Cell host & microbe,
S M Kerr, and L H Johnston, and M Odell, and S A Duncan, and K M Law, and G L Smith
The DNA repair capability of cdc9, the Saccharomyces cerevisiae mutant defective in DNA ligase.
February 1979, Molecular & general genetics : MGG,
S M Kerr, and L H Johnston, and M Odell, and S A Duncan, and K M Law, and G L Smith
DNA ligase I from Saccharomyces cerevisiae: physical and biochemical characterization of the CDC9 gene product.
December 1992, Biochemistry,
S M Kerr, and L H Johnston, and M Odell, and S A Duncan, and K M Law, and G L Smith
An improved assay for DNA ligase reveals temperature-sensitive activity in cdc9 mutants of Saccharomyces cerevisiae.
January 1985, Molecular & general genetics : MGG,
S M Kerr, and L H Johnston, and M Odell, and S A Duncan, and K M Law, and G L Smith
Specific Saccharomyces cerevisiae genes are expressed in response to DNA-damaging agents.
January 1985, Molecular and cellular biology,
S M Kerr, and L H Johnston, and M Odell, and S A Duncan, and K M Law, and G L Smith
The role of the cdc9 ligase in replication and excision repair in Saccharomyces cerevisiae.
October 1982, Current genetics,
S M Kerr, and L H Johnston, and M Odell, and S A Duncan, and K M Law, and G L Smith
The protein degradation response of Saccharomyces cerevisiae to classical DNA-damaging agents.
December 2007, Chemical research in toxicology,
Copied contents to your clipboard!