Travelling waves of attached and detached cells in a wound-healing cell migration assay. 2007

Kerry A Landman, and Anna Q Cai, and Barry D Hughes
Department of Mathematics and Statistics, University of Melbourne, Victoria, 3010, Australia. k.landman@ms.unimelb.edu.au

During a wound-healing cell migration assay experiment, cells are observed to detach and undergo mitosis before reattaching as a pair of cells on the substrate. During experiments with mice 3T3 fibroblasts, cell detachment can be confined to the wavefront region or it can occur over the whole wave profile. A multi-species continuum approach to modelling a wound-healing assay is taken to account for this phenomenon. The first cell population is composed of attached motile cells, while the second population is composed of detached immotile cells undergoing mitosis and returning to the migrating population after successful division. The first model describes cell division occurring only in the wavefront region, while a second model describes cell division over the whole of the scrape wound. The first model reverts to the Fisher equation when the reattachment rate relative to the detachment rate is large, while the second case does not revert to the Fisher equation in any limit. The models yield travelling wave solutions. The minimum wave speed is slower than the minimum Fisher wave speed and its dependence on the cell detachment and reattachment rate parameters is analysed. Approximate travelling wave profiles of the two cell populations are determined asymptotically under certain parameter regimes.

UI MeSH Term Description Entries
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D002448 Cell Adhesion Adherence of cells to surfaces or to other cells. Adhesion, Cell,Adhesions, Cell,Cell Adhesions
D002465 Cell Movement The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell. Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D005347 Fibroblasts Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. Fibroblast
D000465 Algorithms A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task. Algorithm
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D014945 Wound Healing Restoration of integrity to traumatized tissue. Healing, Wound,Healings, Wound,Wound Healings
D016475 3T3 Cells Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION. 3T3 Cell,Cell, 3T3,Cells, 3T3

Related Publications

Kerry A Landman, and Anna Q Cai, and Barry D Hughes
January 2021, Methods in molecular biology (Clifton, N.J.),
Kerry A Landman, and Anna Q Cai, and Barry D Hughes
February 2010, American journal of physiology. Heart and circulatory physiology,
Kerry A Landman, and Anna Q Cai, and Barry D Hughes
March 2018, Journal of visualized experiments : JoVE,
Kerry A Landman, and Anna Q Cai, and Barry D Hughes
August 2013, Mathematical biosciences,
Kerry A Landman, and Anna Q Cai, and Barry D Hughes
January 2011, Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference,
Kerry A Landman, and Anna Q Cai, and Barry D Hughes
January 2004, Tissue engineering,
Kerry A Landman, and Anna Q Cai, and Barry D Hughes
October 2018, Oral diseases,
Kerry A Landman, and Anna Q Cai, and Barry D Hughes
December 2015, Acta biotheoretica,
Kerry A Landman, and Anna Q Cai, and Barry D Hughes
January 2013, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society,
Kerry A Landman, and Anna Q Cai, and Barry D Hughes
July 2016, Biomaterials science,
Copied contents to your clipboard!