Infectivity of herpes simplex virus type-1 (HSV-1) amplicon vectors in dendritic cells is determined by the helper virus strain used for packaging. 2007

Kathlyn Santos, and Christine M Sanfilippo, and Wade C Narrow, and Ann E Casey, and Sol M Rodriguez-Colon, and Michael P McDermott, and Howard J Federoff, and William J Bowers, and Stephen Dewhurst
Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 601 Elmwood Avenue, Box 672, Rochester, NY 14642, USA.

Herpes simplex virus type-1 (HSV-1) amplicon vectors are being explored for a wide range of potential applications, including vaccine delivery and immunotherapy of cancer. While extensive effort has been directed towards the improvement of the amplicon "payload" in these vectors, relatively little attention has been paid to the effect of the packaging HSV-1 strains on the biological properties of co-packaged amplicon vectors. We therefore compared the biological properties of amplicon stocks prepared using a panel of primary HSV-1 isolates, a molecularly cloned strain used to package helper-free amplicons (designated here as F5), and two laboratory isolates (KOS and strain 17, which is the parent of the F5 clone). This analysis revealed considerable inter-strain variability in the ability of amplicon stocks packaged by different primary HSV-1 isolates to efficiently transduce established cell lines and primary human dendritic cells (DC). Amplicons packaged by both the F5 molecularly cloned virus and its laboratory-adapted parent (strain 17) were very inefficient at transducing DC, when compared to amplicons packaged by KOS or by several of the primary virus isolates. These finding have important implications for the future development of improved amplicon-based vaccine delivery systems and suggest that DC tropism may be an instrinsic property of some HSV-1 strains, independent of passage history or molecular cloning.

UI MeSH Term Description Entries
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002522 Chlorocebus aethiops A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research. African Green Monkey,Cercopithecus aethiops,Cercopithecus griseoviridis,Cercopithecus griseus,Cercopithecus pygerythrus,Cercopithecus sabeus,Cercopithecus tantalus,Chlorocebus cynosuros,Chlorocebus cynosurus,Chlorocebus pygerythrus,Green Monkey,Grivet Monkey,Lasiopyga weidholzi,Malbrouck,Malbrouck Monkey,Monkey, African Green,Monkey, Green,Monkey, Grivet,Monkey, Vervet,Savanah Monkey,Vervet Monkey,Savannah Monkey,African Green Monkey,Chlorocebus cynosuro,Green Monkey, African,Green Monkeys,Grivet Monkeys,Malbrouck Monkeys,Malbroucks,Monkey, Malbrouck,Monkey, Savanah,Monkey, Savannah,Savannah Monkeys,Vervet Monkeys
D003713 Dendritic Cells Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION). Dendritic Cells, Interdigitating,Interdigitating Cells,Plasmacytoid Dendritic Cells,Veiled Cells,Dendritic Cells, Interstitial,Dendritic Cells, Plasmacytoid,Interdigitating Dendritic Cells,Interstitial Dendritic Cells,Cell, Dendritic,Cell, Interdigitating,Cell, Interdigitating Dendritic,Cell, Interstitial Dendritic,Cell, Plasmacytoid Dendritic,Cell, Veiled,Cells, Dendritic,Cells, Interdigitating,Cells, Interdigitating Dendritic,Cells, Interstitial Dendritic,Cells, Plasmacytoid Dendritic,Cells, Veiled,Dendritic Cell,Dendritic Cell, Interdigitating,Dendritic Cell, Interstitial,Dendritic Cell, Plasmacytoid,Interdigitating Cell,Interdigitating Dendritic Cell,Interstitial Dendritic Cell,Plasmacytoid Dendritic Cell,Veiled Cell
D005822 Genetic Vectors DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition. Cloning Vectors,Shuttle Vectors,Vectors, Genetic,Cloning Vector,Genetic Vector,Shuttle Vector,Vector, Cloning,Vector, Genetic,Vector, Shuttle,Vectors, Cloning,Vectors, Shuttle
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014162 Transfection The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES. Transfections
D014709 Vero Cells A CELL LINE derived from the kidney of the African green (vervet) monkey, (CHLOROCEBUS AETHIOPS) used primarily in virus replication studies and plaque assays. Cell, Vero,Cells, Vero,Vero Cell
D018259 Herpesvirus 1, Human The type species of SIMPLEXVIRUS causing most forms of non-genital herpes simplex in humans. Primary infection occurs mainly in infants and young children and then the virus becomes latent in the dorsal root ganglion. It then is periodically reactivated throughout life causing mostly benign conditions. HSV-1,Herpes Simplex Virus 1,HHV-1,Herpes Simplex Virus Type 1,Herpesvirus 1 (alpha), Human,Human Herpesvirus 1
D019065 Virus Assembly The assembly of VIRAL STRUCTURAL PROTEINS and nucleic acid (VIRAL DNA or VIRAL RNA) to form a VIRUS PARTICLE. Viral Assembly,Assembly, Viral,Assembly, Virus

Related Publications

Kathlyn Santos, and Christine M Sanfilippo, and Wade C Narrow, and Ann E Casey, and Sol M Rodriguez-Colon, and Michael P McDermott, and Howard J Federoff, and William J Bowers, and Stephen Dewhurst
January 2014, Methods in molecular biology (Clifton, N.J.),
Kathlyn Santos, and Christine M Sanfilippo, and Wade C Narrow, and Ann E Casey, and Sol M Rodriguez-Colon, and Michael P McDermott, and Howard J Federoff, and William J Bowers, and Stephen Dewhurst
January 2020, Methods in molecular biology (Clifton, N.J.),
Kathlyn Santos, and Christine M Sanfilippo, and Wade C Narrow, and Ann E Casey, and Sol M Rodriguez-Colon, and Michael P McDermott, and Howard J Federoff, and William J Bowers, and Stephen Dewhurst
February 2005, Pathologie-biologie,
Kathlyn Santos, and Christine M Sanfilippo, and Wade C Narrow, and Ann E Casey, and Sol M Rodriguez-Colon, and Michael P McDermott, and Howard J Federoff, and William J Bowers, and Stephen Dewhurst
March 2012, Cold Spring Harbor protocols,
Kathlyn Santos, and Christine M Sanfilippo, and Wade C Narrow, and Ann E Casey, and Sol M Rodriguez-Colon, and Michael P McDermott, and Howard J Federoff, and William J Bowers, and Stephen Dewhurst
January 2015, Methods in molecular biology (Clifton, N.J.),
Kathlyn Santos, and Christine M Sanfilippo, and Wade C Narrow, and Ann E Casey, and Sol M Rodriguez-Colon, and Michael P McDermott, and Howard J Federoff, and William J Bowers, and Stephen Dewhurst
October 1996, Human gene therapy,
Kathlyn Santos, and Christine M Sanfilippo, and Wade C Narrow, and Ann E Casey, and Sol M Rodriguez-Colon, and Michael P McDermott, and Howard J Federoff, and William J Bowers, and Stephen Dewhurst
January 2011, Methods in molecular biology (Clifton, N.J.),
Kathlyn Santos, and Christine M Sanfilippo, and Wade C Narrow, and Ann E Casey, and Sol M Rodriguez-Colon, and Michael P McDermott, and Howard J Federoff, and William J Bowers, and Stephen Dewhurst
July 2022, International journal of molecular sciences,
Kathlyn Santos, and Christine M Sanfilippo, and Wade C Narrow, and Ann E Casey, and Sol M Rodriguez-Colon, and Michael P McDermott, and Howard J Federoff, and William J Bowers, and Stephen Dewhurst
August 2005, Gene therapy,
Kathlyn Santos, and Christine M Sanfilippo, and Wade C Narrow, and Ann E Casey, and Sol M Rodriguez-Colon, and Michael P McDermott, and Howard J Federoff, and William J Bowers, and Stephen Dewhurst
September 1998, Journal of virology,
Copied contents to your clipboard!