BIOMAT Database Logo BIOMAT Database Logo

[Simple method of "mini-prep": small scale preparation of plasmids from E. coli]. 1991

M Usami, and W Yamao-Harigaya, and K Maruyama
Department of Molecular Biology, Psychiatric Research Institute of Tokyo.

UI MeSH Term Description Entries
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D001709 Biotechnology Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction. Biotechnologies

Related Publications

M Usami, and W Yamao-Harigaya, and K Maruyama
[A rapid, simple and inexpensive preparation method of small amount, but large numbers of E. coli plasmids].
January 1994, Seikagaku. The Journal of Japanese Biochemical Society,
M Usami, and W Yamao-Harigaya, and K Maruyama
A simple single-step procedure for small-scale preparation of Escherichia coli plasmids.
March 1990, Nucleic acids research,
M Usami, and W Yamao-Harigaya, and K Maruyama
Mini-chromosomes: plasmids which carry the E. coli replication origin.
July 1978, Molecular & general genetics : MGG,
M Usami, and W Yamao-Harigaya, and K Maruyama
[Isolation of phPAH 247 cDNA probe from pBR322 plasmids by mini-prep technique].
April 1992, Mikrobiyoloji bulteni,
M Usami, and W Yamao-Harigaya, and K Maruyama
The CTAB-DNA precipitation method: a common mini-scale preparation of template DNA from phagemids, phages or plasmids suitable for sequencing.
May 1989, BioTechniques,
M Usami, and W Yamao-Harigaya, and K Maruyama
A simple method for the preparation of plasmid and chromosomal E. coli DNA.
November 1989, Nucleic acids research,
M Usami, and W Yamao-Harigaya, and K Maruyama
Double-stranded sequencing, using mini-prep plasmids, in eleven hours.
January 1988, BioTechniques,
M Usami, and W Yamao-Harigaya, and K Maruyama
RNA isolation: a mini-prep method.
November 1988, Nucleic acids research,
M Usami, and W Yamao-Harigaya, and K Maruyama
A new method of large scale preparation of highly purified DNA-dependent RNA-polymerase from E. coli.
February 1970, Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
M Usami, and W Yamao-Harigaya, and K Maruyama
Transfer of small YACs to E. coli as large circular plasmids.
July 1997, Genetic analysis : biomolecular engineering,
Copied contents to your clipboard!