-transparent.png){kind=logo}
We have isolated plasmids by linking the 5.9 MD EcoRI fragment of E. coli that carries the origin of replication to an EcoRI fragment that carries an amplicillin resistance determinant, but lacks an origin of replication. 3 plasmids of this type, pOC1, pOC2, and pOC3, are described in detail in this report. Although the plasmids have some adverse effect on the growth properties of the host strain, their existence shows that two functioning chromosomal origins can coexist in one cell. Deletions generated from this type of plasmids allow an allocation of the origin of replication of E. coli within a DNA segment less than 0.4 MD in size.
Related Publications
W Messer, and
H E Bergmans, and
M Meijer, and
J E Womack, and
F G Hansen, and
K von Meyenburg
November 1981,
Nature,
W Messer, and
H E Bergmans, and
M Meijer, and
J E Womack, and
F G Hansen, and
K von Meyenburg
July 1987,
Cell,
W Messer, and
H E Bergmans, and
M Meijer, and
J E Womack, and
F G Hansen, and
K von Meyenburg
June 1984,
Journal of molecular biology,
W Messer, and
H E Bergmans, and
M Meijer, and
J E Womack, and
F G Hansen, and
K von Meyenburg
September 1982,
Proceedings of the National Academy of Sciences of the United States of America,
W Messer, and
H E Bergmans, and
M Meijer, and
J E Womack, and
F G Hansen, and
K von Meyenburg
October 2003,
Journal of bacteriology,
W Messer, and
H E Bergmans, and
M Meijer, and
J E Womack, and
F G Hansen, and
K von Meyenburg
January 1984,
Cell,
W Messer, and
H E Bergmans, and
M Meijer, and
J E Womack, and
F G Hansen, and
K von Meyenburg
April 1986,
The Journal of biological chemistry,
W Messer, and
H E Bergmans, and
M Meijer, and
J E Womack, and
F G Hansen, and
K von Meyenburg
June 1978,
Molecular & general genetics : MGG,
W Messer, and
H E Bergmans, and
M Meijer, and
J E Womack, and
F G Hansen, and
K von Meyenburg
January 1981,
Molecular & general genetics : MGG,
W Messer, and
H E Bergmans, and
M Meijer, and
J E Womack, and
F G Hansen, and
K von Meyenburg
November 1991,
Seikagaku. The Journal of Japanese Biochemical Society,
