Biomaterial Database

A comparative efficacy of plate ELISA and dot ELISA to detect antiamoebic antibodies in clinical patients. 1991

J R Kanwar, and V K Vinayak

Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

The dot enzyme-linked immunosorbent assay (dot ELISA) has been developed and compared with plate ELISA for detecting antiamoebic antibodies in serum samples from amoebic patients and healthy controls. Both dot and plate ELISA detected antiamoebic antibodies in all 17 confirmed amoebic liver abscess cases and 15 (68%) of 22 suspected amoebic liver abscess cases while none of 21 non-amoebic hepatic disorders, 25 non-amoebic intestinal disorders and 28 apparently healthy subjects had antiamoebic antibodies as assessed by either of the tests. All the 15 suspected amoebic liver abscess patients who had antiamoebic antibodies responded favourably to antiamoebic treatment. As the dot ELISA is simpler, rapid, inexpensive, equally sensitive and specific as compared to plate ELISA, its use in detecting antiamoebic antibodies is recommended especially under field conditions.

UI	MeSH Term	Description	Entries
D008101	Liver Abscess, Amebic	Single or multiple areas of PUS due to infection by any ameboid protozoa (AMEBIASIS). A common form is caused by the ingestion of ENTAMOEBA HISTOLYTICA.	Abscess, Amebic, Hepatic,Abscess, Hepatic, Amebic,Abscess, Liver, Amebic,Amebiasis, Hepatic,Entamoebiasis, Hepatic,Hepatic Amebiasis,Hepatic Entamoebiasis,Amoebiasis, Hepatic,Hepatic Abscess, Amoebic,Hepatic Amoebiasis,Liver Abscess, Amoebic,Abscess, Amebic Liver,Abscess, Amoebic Hepatic,Abscess, Amoebic Liver,Abscesses, Amebic Liver,Abscesses, Amoebic Hepatic,Abscesses, Amoebic Liver,Amebic Liver Abscess,Amebic Liver Abscesses,Amoebic Hepatic Abscess,Amoebic Hepatic Abscesses,Amoebic Liver Abscess,Amoebic Liver Abscesses,Entamoebiases, Hepatic,Hepatic Abscesses, Amoebic,Hepatic Entamoebiases,Liver Abscesses, Amebic,Liver Abscesses, Amoebic
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005069	Evaluation Studies as Topic	Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies.	Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000913	Antibodies, Protozoan	Immunoglobulins produced in a response to PROTOZOAN ANTIGENS.	Protozoan Antibodies
D012680	Sensitivity and Specificity	Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)	Specificity,Sensitivity,Specificity and Sensitivity
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor