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Development of a comparative dot ELISA for the detection of antibodies in blastomycosis. 1987

H Sato, and A S Honigman, and G M Scalarone
Department of Biological Sciences, Idaho State University, Pocatello 83209.

We present a preliminary study utilizing an enzyme immunoassay for the detection of antibodies in blastomycosis using nitrocellulose membrane as the solid support. The comparative dot enzyme-linked immunosorbent assay (CDE) utilizing Blastomyces dermatitidis, Histoplasma capsulatum, and Coccidioides immitis antigens allows rapid screening of specimens and determination of a serum profile with respect to anti-Blastomyces antibodies. Sensitivity (76% or greater) and specificity (approximately 12% crossreactivity) determinations indicated that this visual assay may be an alternative antibody detection method for blastomycosis or other systemic fungal diseases.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D001759 Blastomycosis A fungal infection that may appear in two forms: 1, a primary lesion characterized by the formation of a small cutaneous nodule and small nodules along the lymphatics that may heal within several months; and 2, chronic granulomatous lesions characterized by thick crusts, warty growths, and unusual vascularity and infection in the middle or upper lobes of the lung. Blastomycosis, North American,Gilchrist Disease,Gilchrist's Disease,Blastomycoses,Gilchrists Disease,North American Blastomycosis
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000908 Antibodies, Fungal Immunoglobulins produced in a response to FUNGAL ANTIGENS. Fungal Antibodies
D000946 Antigens, Fungal Substances of fungal origin that have antigenic activity. Fungal Antigen,Fungal Antigens,Antigen, Fungal

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