-transparent.png){kind=logo}
Scrapie is a naturally occurring fatal neurodegenerative disease of sheep and goats. This study documents incubation periods, pathologic findings, and distribution of abnormal prion proteins (PrP(Sc)) by immunohistochemistry in tissues of genetically susceptible sheep inoculated with US sheep scrapie agent. Four-month-old Suffolk lambs (QQ at codon 171) were inoculated by 1 of 3 different routes (nasal, peritoneal, and conjunctival) with an inoculum (No. 13-7) consisting of a pool of scrapie-affected sheep brains. Except for 3 sheep, all inoculated animals were euthanized when advanced clinical signs of scrapie were observed between 19 and 46 months postinoculation (MPI). Spongiform lesions in the brains and labeling of PrP(Sc) in central nervous system and lymphoid tissues were present in these sheep. One intranasally inoculated sheep euthanized at 12 MPI had presence of PrP(Sc) that was confined to the pharyngeal tonsil. These results indicate that the upper respiratory tract, specifically the pharyngeal tonsil, may serve as a portal of entry for prion protein in scrapie-infected environments.
A N Hamir, and
R A Kunkle, and
J A Richt, and
J M Miller, and
J J Greenlee
October 2020,
Research in veterinary science,
A N Hamir, and
R A Kunkle, and
J A Richt, and
J M Miller, and
J J Greenlee
August 2007,
BMC veterinary research,
A N Hamir, and
R A Kunkle, and
J A Richt, and
J M Miller, and
J J Greenlee
January 2000,
In vitro cellular & developmental biology. Animal,
A N Hamir, and
R A Kunkle, and
J A Richt, and
J M Miller, and
J J Greenlee
October 2002,
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire,
A N Hamir, and
R A Kunkle, and
J A Richt, and
J M Miller, and
J J Greenlee
May 2011,
Emerging infectious diseases,
A N Hamir, and
R A Kunkle, and
J A Richt, and
J M Miller, and
J J Greenlee
January 2020,
PloS one,
A N Hamir, and
R A Kunkle, and
J A Richt, and
J M Miller, and
J J Greenlee
October 2011,
Veterinary research,
A N Hamir, and
R A Kunkle, and
J A Richt, and
J M Miller, and
J J Greenlee
September 2002,
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
A N Hamir, and
R A Kunkle, and
J A Richt, and
J M Miller, and
J J Greenlee
January 2011,
PloS one,
A N Hamir, and
R A Kunkle, and
J A Richt, and
J M Miller, and
J J Greenlee
April 1965,
Journal of comparative pathology,
